Sakakibara Y, Tsukano H, Sako T
Gene. 1981 Jan-Feb;13(1):47-55. doi: 10.1016/0378-1119(81)90042-1.
The locations of the linked dnaA and dnaN genes of Escherichia coli in a specialized transducing lambda phage genome have been determined by electron microscopic heteroduplex analysis, using phages with deletions or insertions in the dnaA or dnaN gene. The transcription initiation sites for the dna genes were also localized by electron microscopic analysis of DNA-RBA heteroduplex molecules formed between the E. coli DNA fragment of the phage genome and the in vitro transcription products of the fragment. The dnaN gene was found to be transcribed in the same direction as the dnaA gene, and predominantly from the promoter of the dnaA gene.
利用在dnaA或dnaN基因中有缺失或插入的噬菌体，通过电子显微镜异源双链分析，已确定了大肠杆菌中相连的dnaA和dnaN基因在一个专门转导的λ噬菌体基因组中的位置。通过对噬菌体基因组的大肠杆菌DNA片段与该片段的体外转录产物之间形成的DNA-RNA异源双链分子进行电子显微镜分析，也确定了dna基因的转录起始位点。发现dnaN基因与dnaA基因沿相同方向转录，且主要从dnaA基因的启动子转录。
