Cieśla Z, Jonczyk P
Mol Gen Genet. 1980;180(3):617-20. doi: 10.1007/BF00268069.
Exposure of Escherichia coli dnaA strains to UV light results in a transient resumption of chromosome replication at 42 degrees C, the temperature restrictive to these mutants. Capability of dnaA mutants to replicate DNA at 42 degrees C can be stabilized, however, when either protein or RNA synthesis is inhibited 60 min after UV irradiation. DNA synthesis proceeds for several hours under these conditions. These results indicate that dnaA dependent transcription is not involved in initiation of chromosome cycles during stable DNA synthesis.
将大肠杆菌dnaA菌株暴露于紫外线下会导致在42℃（这些突变体的温度限制条件）下染色体复制短暂恢复。然而，当在紫外线照射60分钟后抑制蛋白质或RNA合成时，dnaA突变体在42℃下复制DNA的能力可以得到稳定。在这些条件下，DNA合成会持续数小时。这些结果表明，在稳定的DNA合成过程中，dnaA依赖性转录不参与染色体循环的起始。
