Kanelis V, Forman-Kay J D, Kay L E
Program in Structural Biology and Biochemistry, Hospital for Sick Children, Toronto, Ontario, Canada.
IUBMB Life. 2001 Dec;52(6):291-302. doi: 10.1080/152165401317291147.
Structural studies of proteins are critical for understanding biological processes at the molecular level. Nuclear magnetic resonance (NMR) spectroscopy is a powerful technique for obtaining structural and dynamic information on proteins and protein-ligand complexes. In the present review, methodologies for NMR structure determination of proteins and macromolecular complexes are described. In addition, a number of recent advances that reduce the molecular weight limitations previously imposed on NMR studies of biomolecules are discussed, highlighting applications of these technologies to protein systems studied in our laboratories.
蛋白质的结构研究对于在分子水平上理解生物过程至关重要。核磁共振（NMR）光谱学是一种获取蛋白质及蛋白质-配体复合物结构和动力学信息的强大技术。在本综述中，描述了蛋白质和大分子复合物的NMR结构测定方法。此外，还讨论了一些近期的进展，这些进展减少了先前对生物分子NMR研究施加的分子量限制，并重点介绍了这些技术在我们实验室所研究的蛋白质系统中的应用。
