Griesinger Christian, Peti Wolfgang, Meiler Jens, Brüschweiler Rafael
Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
Methods Mol Biol. 2004;278:107-21. doi: 10.1385/1-59259-809-9:107.
This chapter presents a methodology that allows for the structural and dynamic characterization of biomolecules by means of projection restraints obtained from residual magnetic dipolar couplings. Dipolar couplings reflect the projection of individual internuclear vectors onto the alignment tensor. This technique allows determination of the dynamics of the protein backbone on time-scales, namely, between the rotational tumbling correlation time and approx 50 micros. This range of time-scales has been previously inaccessible by other nuclear magnetic resonance (NMR) techniques. In addition, information about the anisotropy of the motion is obtained.
本章介绍了一种方法，该方法可通过从残余磁偶极耦合获得的投影约束来对生物分子进行结构和动力学表征。偶极耦合反映了各个核间矢量在取向张量上的投影。该技术能够确定蛋白质主链在时间尺度上的动力学，即介于旋转翻滚相关时间和约50微秒之间。此前，其他核磁共振（NMR）技术无法获取这一时间尺度范围。此外，还能获得有关运动各向异性的信息。
