Wang Yui-Hsi, Stephan Robert P, Scheffold Alexander, Kunkel Désirée, Karasuyama Hajime, Radbruch Andreas, Cooper Max D
Department of Medicine, Division of Developmental and Clinical Immunology, University of Alabama at Birmingham, 1824 6th Avenue S, Birmingham, AL 35294-3300, USA.
Blood. 2002 Apr 1;99(7):2459-67. doi: 10.1182/blood.v99.7.2459.
Surrogate light chain expression during B lineage differentiation was examined by using indicator fluorochrome-filled liposomes in an enhanced immunofluorescence assay. Pro-B cells bearing surrogate light chain components were found in mice, but not in humans. A limited subpopulation of relatively large pre-B cells in both species expressed pre-B cell receptors. These cells had reduced expression of the recombinase activating genes, RAG-1 and RAG-2. Their receptor-negative pre-B cell progeny were relatively small, expressed RAG-1 and RAG-2, and exhibited selective down-regulation of VpreB and lambda5 expression. Comparative analysis of the 2 pre-B cell subpopulations indicated that loss of the pre-B cell receptors from surrogate light chain gene silencing was linked with exit from the cell cycle and light chain gene rearrangement to achieve B-cell differentiation.
在增强免疫荧光测定中，通过使用填充有指示荧光染料的脂质体来检测B细胞谱系分化过程中的替代轻链表达。在小鼠中发现了携带替代轻链成分的前B细胞，但在人类中未发现。两个物种中相对较大的前B细胞的有限亚群表达前B细胞受体。这些细胞中重组激活基因RAG-1和RAG-2的表达降低。它们的受体阴性前B细胞后代相对较小，表达RAG-1和RAG-2，并表现出VpreB和lambda5表达的选择性下调。对这两个前B细胞亚群的比较分析表明，替代轻链基因沉默导致前B细胞受体的丧失与退出细胞周期和轻链基因重排以实现B细胞分化有关。
