Li Qian, Han Dongmei, Wang Wei, Liu Xiaoqing, Sun Xiuyuan, Zhang Jun, Li Rong, Zhang Yu
Department of Immunology, Key Laboratory of Medical Immunology of Ministry of Health, Peking University Health Science Center, Beijing, China.
Department of Microbiology, Faculty of Basic Medical Sciences, Institute of Translational Medicine, Nanchang University, Nanchang, China.
Cell Mol Immunol. 2014 Mar;11(2):132-40. doi: 10.1038/cmi.2013.55. Epub 2013 Dec 9.
Lipopolysaccharide (LPS) is known to be a potent activator of mature B cells by signaling through Toll-like receptor 4 (TLR4). Its impact on early B-cell development, however, is not well defined. When comparing to C3H/HeN mice, TLR4-mutant C3H/HeJ mice showed an increase in the number of pro-B and pre-B cells in the bone marrow. When cultured in the presence of IL-7, the proliferation of pro-B and large pre-B cells was significantly inhibited by LPS, possibly due to reduced IL-7 receptor-α (IL-7Rα) expression. Meanwhile, the generation of IgM(+)/IgD(+) B cells was greatly enhanced in IL-7 cultures of pro-B and pre-B cells. Consistent with these results, treatment with LPS facilitated the progression of adoptively transferred B220(+)IgM(-)IgD(-) precursors into IgD(+) cells. Overall, these data suggest that LPS has a profound influence on early B-cell development, which may contribute to the deregulated B-cell development under physiological and pathological conditions such as bacterial infections.
已知脂多糖（LPS）通过Toll样受体4（TLR4）信号传导，是成熟B细胞的有效激活剂。然而，其对早期B细胞发育的影响尚不清楚。与C3H/HeN小鼠相比，TLR4突变的C3H/HeJ小鼠骨髓中前B细胞和前B细胞数量增加。在IL-7存在下培养时，LPS显著抑制前B细胞和大前B细胞的增殖，这可能是由于IL-7受体α（IL-7Rα）表达降低所致。同时，在前B细胞和前B细胞的IL-7培养物中，IgM(+)/IgD(+) B细胞的生成大大增强。与这些结果一致，LPS处理促进了过继转移的B220(+)IgM(-)IgD(-)前体细胞向IgD(+)细胞的进展。总体而言，这些数据表明LPS对早期B细胞发育有深远影响，这可能导致在生理和病理条件如细菌感染下B细胞发育失调。
