Ghia P, ten Boekel E, Sanz E, de la Hera A, Rolink A, Melchers F
Basel Institute for Immunology, Switzerland.
J Exp Med. 1996 Dec 1;184(6):2217-29. doi: 10.1084/jem.184.6.2217.
CD19+CD10+ human B lineage bone marrow cells were separated into cycling or resting cells, which differ in their expression of CD34, VpreB, recombination activating gene (RAG-1), and terminal deoxynucleotidyl transferase (TdT). Polymerase chain reaction analyses developed for DHJH and VkJk, VkJkK(de) and VkK(de) rearrangements with DNA of single cells and a comparison with B lineage cell development in mouse bone marrow, allow to delineate the human B lymphocyte pathway of development as follows: CD34+VpreB+RAG-1+TdT+, DHJH-rearranged, kL germline cycling pre-B I cells-->CD34-VpreB+microH chain+ (pre-B receptor+) RAG-1-TdT-, VHDHJH-rearranged, kL germline, cycling pre-B II cells-->CD34-VpreB-, intracytoplasmic microH chain+ (pre-B receptor-) RAG-1+/-TdT-, VHDHJH-rearranged, mainly kL germline cycling pre-B II cells-->CD34-VpreB-intracytoplasmic microH chain+, RAG-1+TdT-, VHDHJH-rearranged, VkJk-rearranged, IgM-, resting pre-B II cells CD34+VpreB-, sIgM+, RAG-1+TdT-, VHDHJH- and VkJk-rearranged IgM+ immature B cells-->CD34-, CD10-, sIgM+/sIgD+ mature B cells. This order, for the first time established for human B lineage cells, shows striking similarities with that established for mouse B lineage cells in bone marrow.
CD19+CD10+人B淋巴细胞系骨髓细胞被分离为循环细胞或静止细胞，它们在CD34、VpreB、重组激活基因（RAG-1）和末端脱氧核苷酸转移酶（TdT）的表达上存在差异。利用单细胞DNA对DHJH和VkJk、VkJkK（de）和VkK（de）重排进行的聚合酶链反应分析，以及与小鼠骨髓中B淋巴细胞系发育的比较，使得人类B淋巴细胞发育途径得以如下描述：CD34+VpreB+RAG-1+TdT+、DHJH重排、κ轻链基因座种系的循环前B I细胞→CD34-VpreB+μ重链+（前B细胞受体+）RAG-1-TdT-、VHDHJH重排、κ轻链基因座种系的循环前B II细胞→CD34-VpreB-、胞浆内μ重链+（前B细胞受体-）RAG-1+/-TdT-、VHDHJH重排、主要是κ轻链基因座种系的循环前B II细胞→CD34-VpreB-胞浆内μ重链+、RAG-1+TdT-、VHDHJH重排、VkJk重排、IgM-的静止前B II细胞→CD34+VpreB-、sIgM+、RAG-1+TdT-、VHDHJH和VkJk重排的IgM+未成熟B细胞→CD34-、CD10-、sIgM+/sIgD+成熟B细胞。这一首次为人B淋巴细胞系细胞确立的顺序，与在小鼠骨髓中为B淋巴细胞系细胞确立的顺序显示出惊人的相似性。
