Soares Colin R, Shibata Masa-Aki, Green Jeffrey E, Jorcyk Cheryl L
Department of Biology, Boise State University, Boise, ID 83725, USA.
Neoplasia. 2002 Mar-Apr;4(2):112-20. doi: 10.1038/sj.neo.7900210.
Existing prostate cancer cell lines have been derived from late stages of human prostate cancer. In this paper, we present two cell lines generated from prostatic intraepithelial neoplasia (PIN), the precursor lesion for prostate adenocarcinoma. Pr-111 and Pr-117 were established from PIN lesions that developed in the C3(1)/Tag transgenic model of prostate cancer. Pr-111 and Pr-117 cells express simian virus 40 large T antigen (SV40 Tag) and are immortalized in culture, distinguishing them from normal prostate cells. The growth rates of these two cell lines are quite different; with Pr-111 cells growing much more slowly (doubling time approximately 40 hours) compared to Pr-117 cells (doubling time approximately 22 hours), and also show significantly different growth rates in different media. Both prostate cell lines express cytokeratin and androgen receptor (AR) with Pr-111 cells demonstrating androgen-dependent growth and Pr-117 cells exhibiting androgen-responsive growth characteristics. Athymic nude mice injected with Pr-111 cells either do not develop tumors or develop tumors after a long latency period of 14 weeks. Pr-117 cells, however, develop tumors by 3 to 6 weeks, suggesting that Pr-117 cells represent a later stage of tumor progression. These two novel cell lines will be useful for studying early stages of prostate tumor development and androgen responsiveness.
现有的前列腺癌细胞系源自人类前列腺癌的晚期阶段。在本文中，我们展示了两种由前列腺上皮内瘤变（PIN）产生的细胞系，前列腺上皮内瘤变是前列腺腺癌的前体病变。Pr - 111和Pr - 117是从前列腺癌C3(1)/Tag转基因模型中发生的PIN病变建立的。Pr - 111和Pr - 117细胞表达猿猴病毒40大T抗原（SV40 Tag），并在培养中永生化，这使它们与正常前列腺细胞区分开来。这两种细胞系的生长速率差异很大；与Pr - 117细胞（倍增时间约22小时）相比，Pr - 111细胞生长要慢得多（倍增时间约40小时），并且在不同培养基中也显示出显著不同的生长速率。两种前列腺细胞系均表达细胞角蛋白和雄激素受体（AR），Pr - 111细胞表现出雄激素依赖性生长，Pr - 117细胞表现出雄激素反应性生长特征。注射Pr - 111细胞的无胸腺裸鼠要么不发生肿瘤，要么在长达14周的潜伏期后发生肿瘤。然而，Pr - 117细胞在3至6周内发生肿瘤，这表明Pr - 117细胞代表肿瘤进展的后期阶段。这两种新的细胞系将有助于研究前列腺肿瘤发生的早期阶段和雄激素反应性。
