Wang M, Liu A, Garcia F U, Rhim J S, Stearns M E
Department of Pathology and Laboratory Medicine, MCP-Hahnemann University, Philadelphia, PA 19102-1192, USA.
Int J Oncol. 1999 Jun;14(6):1185-95. doi: 10.3892/ijo.14.6.1185.
Cultures from high grade prostatic intraepithelial neoplasia (HGPIN) have been established and immortalized by HPV-18 infection. The cultures were identified as PIN by Western blotting with anti-cytokeratin (34betaE12) and prostate specific antigen (PSA) antibodies. We examined the growth capabilities of the cultures in the presence of TGF-beta1, activin-A, follistatin (FS), androgens (DHEA, DHT) and several cytokines (IL-10, IL-2, IL-4). IL-10, FS, and DHT stimulated cell proliferation and colony forming ability, while the other cytokines and growth factors had no discernable effect. In addition, DHT and to a lesser extent IL-10 both stimulated PSA production. Activin-A blocked IL-10, FS, and DHT stimulated growth and PSA production. We interpret the data to mean that IL-10 induction of FS secretion (and FS binding of activin A) restores the normal growth capabilities of HGPIN cultures.
来自高级别前列腺上皮内瘤变（HGPIN）的培养物已通过人乳头瘤病毒18型（HPV - 18）感染建立并永生化。通过使用抗细胞角蛋白（34βE12）和前列腺特异性抗原（PSA）抗体进行蛋白质免疫印迹法，将这些培养物鉴定为PIN。我们检测了在转化生长因子β1（TGF - β1）、激活素A、卵泡抑素（FS）、雄激素（脱氢表雄酮、双氢睾酮）和几种细胞因子（白细胞介素10、白细胞介素2、白细胞介素4）存在的情况下这些培养物的生长能力。白细胞介素10、卵泡抑素和双氢睾酮刺激细胞增殖和集落形成能力，而其他细胞因子和生长因子没有明显作用。此外，双氢睾酮以及在较小程度上白细胞介素10均刺激PSA的产生。激活素A阻断白细胞介素10、卵泡抑素和双氢睾酮刺激的生长和PSA产生。我们将这些数据解释为白细胞介素10诱导卵泡抑素分泌（以及卵泡抑素与激活素A结合）可恢复HGPIN培养物的正常生长能力。
