Cheng Shi-Bin, Kuchiiwa Satoshi, Nagatomo Itsugi, Akasaki Yasuaki, Uchida Masahiro, Tominaga Masataka, Hashiguchi Wataru, Kuchiiwa Toshiko, Nakagawa Shiro
Department of Anatomy, Faculty of Medicine, Kagoshima University, Sakuragaoka 8-35-1, 890-8520, Japan.
Brain Res. 2002 Mar 29;931(2):176-80. doi: 10.1016/s0006-8993(02)02257-6.
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is one of the most toxic environmental pollutants. In the present study, we examined c-Fos expression in the central nervous system (CNS) after administration of a lethal dose of TCDD to the adult Long-Evans rat to clarify if the CNS participates in TCDD-induced intoxication. A single dose of TCDD (dissolved in olive oil, 50 microg/kg) or olive oil alone was administered to the rats by gavage. Animals were allowed to survive for 1 day to 5 weeks. Three days after the administration, a significantly large number of Fos-immunopositive cells were found in the hypothalamus (i.e. dorsomedial hypothalamic nucleus, paraventricular hypothalamic nucleus, medial preoptic nucleus), central amygdaloid nucleus and bed nucleus of the stria terminalis. These results suggest that some TCDD toxicity may be induced by its direct action on the CNS.
2,3,7,8-四氯二苯并对二恶英（TCDD）是毒性最强的环境污染物之一。在本研究中，我们给成年Long-Evans大鼠施用致死剂量的TCDD后，检测其中枢神经系统（CNS）中的c-Fos表达，以阐明CNS是否参与TCDD诱导的中毒过程。通过灌胃给大鼠单次施用TCDD（溶于橄榄油，50微克/千克）或仅施用橄榄油。让动物存活1天至5周。给药后三天，在下丘脑（即背内侧下丘脑核、室旁下丘脑核、内侧视前核）、中央杏仁核和终纹床核中发现大量Fos免疫阳性细胞。这些结果表明，TCDD的某些毒性可能是由其对CNS的直接作用所诱导的。
