黑腹果蝇功能性神经肽F受体的特性分析
Characterization of a functional neuropeptide F receptor from Drosophila melanogaster.
作者信息
Garczynski Stephen F, Brown Mark R, Shen Ping, Murray Thomas F, Crim Joe W
机构信息
Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
出版信息
Peptides. 2002 Apr;23(4):773-80. doi: 10.1016/s0196-9781(01)00647-7.
Potential receptors for Drosophila neuropeptide F (DmNPF) were identified in the genome database. One receptor (DmNPFR1) sequence resembled the Lymnaea NPY receptor, an invertebrate homolog of the vertebrate Y-receptor family. DmNPFR1 was cloned and tested for functionality in stably transfected mammalian CHO cells. In whole cell binding assays, DmNPF displaced 125I-NPF in a concentration-dependent manner (IC(50) = 65 nM). DmNPF inhibited forskolin-stimulated adenylyl cyclase activity similarly (IC(50) = 51 nM). Whole-mount in situ hybridization revealed that DmNPFR1 RNA is expressed in CNS and midgut of Drosophila larvae. DmNPFR1, a new invertebrate Y-receptor homolog, apparently is a functional receptor for DmNPF.
在基因组数据库中鉴定出了果蝇神经肽F（DmNPF）的潜在受体。一种受体（DmNPFR1）序列类似于椎实螺NPY受体，它是脊椎动物Y受体家族的无脊椎动物同源物。克隆了DmNPFR1，并在稳定转染的哺乳动物CHO细胞中测试其功能。在全细胞结合试验中，DmNPF以浓度依赖的方式取代125I-NPF（IC50 = 65 nM）。DmNPF同样抑制福斯高林刺激的腺苷酸环化酶活性（IC50 = 51 nM）。整装原位杂交显示，DmNPFR1 RNA在果蝇幼虫的中枢神经系统和中肠中表达。DmNPFR1是一种新的无脊椎动物Y受体同源物，显然是DmNPF的功能性受体。
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