School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, South Korea.
Laboratory of G Protein Coupled Receptors, Graduate School of Medicine, Korea University, Seoul, South Korea.
FEBS Lett. 2014 Jun 5;588(12):2037-41. doi: 10.1016/j.febslet.2014.04.028. Epub 2014 May 3.
To identify ligands for orphan GPCRs, we searched novel neuropeptide genes in the Drosophila melanogaster genome. Here, we describe CNMa, a novel cyclic neuropeptide that is a highly potent and selective agonist for the orphan GPCR, CG33696 (CNMaR). Phylogenetic analysis revealed that arthropod species have two paralogous CNMaRs, but many taxa retain only one. Drosophila CNMa potently activates CNMaR-2 from Apis mellifera, suggesting both receptors are functional. Although CNMa is conserved in most arthropods, Lepidoptera lack the CNMa gene. However, they retain the CNMaR gene. Bombyx CNMaR showed low sensitivity to Drosophila CNMa, hinting toward the existence of additional CNMaR ligand(s).
为了鉴定孤儿 G 蛋白偶联受体的配体，我们在黑腹果蝇基因组中搜索新的神经肽基因。在这里，我们描述了 CNMa，这是一种新型的环状神经肽，对孤儿 G 蛋白偶联受体 CG33696（CNMaR）具有高度的效力和选择性。系统发生分析表明，节肢动物物种具有两个同源的 CNMaR，但许多分类群只保留一个。果蝇 CNMa 强烈激活来自蜜蜂的 CNMaR-2，表明这两个受体都是功能性的。虽然 CNMa 在大多数节肢动物中是保守的，但鳞翅目缺乏 CNMa 基因。然而，它们保留了 CNMaR 基因。蚕 CNMaR 对果蝇 CNMa 的敏感性较低，这暗示存在额外的 CNMaR 配体。
