Martínez-Pérez Francisco, Valdés Jesús, Zinker Samuel, Aréchiga Hugo
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados, I.P.N., México, D. F, Mexico City, Mexico.
Peptides. 2002 Apr;23(4):781-6. doi: 10.1016/s0196-9781(01)00648-9.
The open reading frame (ORF) of the gene for the precursor of the octapeptide Red Pigment Concentrating Hormone (RPCH) from the blue crab Callinectes sapidus was cloned by PCR with oligonucleotides targeted to the initiation and the end of the translation coding sequences. A 272 bp intron was characterized between nucleotides 343 and 344 of the reported cDNA, present in the region coding for the last amino acids of the precursor related peptide of RPCH. The intron genomic structure here described is similar to that reported for the gene coding for the Adipokinetic Hormone (AKH) of the grasshopper Schistocerca nitans.
利用针对翻译编码序列起始和末端的寡核苷酸，通过聚合酶链式反应（PCR）克隆了来自青蟹（Callinectes sapidus）的八肽红色素浓缩激素（RPCH）前体基因的开放阅读框（ORF）。在所报道的cDNA的核苷酸343和344之间鉴定出一个272 bp的内含子，该内含子存在于编码RPCH前体相关肽最后几个氨基酸的区域。这里描述的内含子基因组结构与报道的沙漠蝗（Schistocerca nitans）脂肪动激素（AKH）编码基因的结构相似。
