Klein J M, Mohrherr C J, Sleutels F, Jaenecke N, Riehm J P, Rao K R
Department of Cellular and Molecular Biology, University of West Florida, Pensacola 32514-5751, USA.
Biochem Biophys Res Commun. 1995 Jul 6;212(1):151-8. doi: 10.1006/bbrc.1995.1949.
A cDNA library was established from the eyestalk ganglia of the blue crab Callinectes sapidus. One clone was isolated (644 bp excluding the poly(A) tail) which encodes the red pigment-concentrating hormone (RPCH)-precursor, consisting of the 25 amino acid residue signal peptide, the RPCH, and a 73 amino acid residue RPCH-precursor related peptide. This clone displays high sequence similarity with a clone isolated from an eyestalk cDNA library of the shore crab Carcinus maenas, in accordance with the close phylogenetic relationship between these species. Northern blot experiments indicated the presence of two different mRNA transcripts which hybridized with a specific RPCH-cDNA probe pointing to the possibility of multiple RPCH isoforms in the blue crab. Although crustacean RPCH and the insect adipokinetic hormones (AKH) are structurally related, their precursors show little similarity.
从青蟹（Callinectes sapidus）的眼柄神经节建立了一个cDNA文库。分离出一个克隆（不包括聚腺苷酸尾为644 bp），其编码红色素浓缩激素（RPCH）前体，由25个氨基酸残基的信号肽、RPCH和一个73个氨基酸残基的RPCH前体相关肽组成。该克隆与从岸蟹（Carcinus maenas）眼柄cDNA文库中分离出的一个克隆显示出高度的序列相似性，这与这些物种之间密切的系统发育关系一致。Northern印迹实验表明存在两种不同的mRNA转录本，它们与特定的RPCH-cDNA探针杂交，表明青蟹中可能存在多种RPCH同工型。尽管甲壳类动物的RPCH和昆虫的脂肪动激素（AKH）在结构上相关，但它们的前体显示出很少的相似性。
