Athman Rafika, Tsocas Annick, Presset Olivier, Robine Sylvie, Rozé Claude, Ferrary Evelyne
Institut Curie, UMR 144, 75248 Paris cedex 05, France.
Am J Physiol Gastrointest Liver Physiol. 2002 Apr;282(4):G634-9. doi: 10.1152/ajpgi.00327.2001.
This study was done to establish and validate a single-pass perfusion method for measuring the absorption of water and electrolytes by the mouse small intestine. The method was then used to study intestinal absorption in mice whose villin gene had been invalidated (v-/-). The single-pass perfusion of the jejunum measures the absorption of water, Cl(-), Na(+), K(+), HCO, and glucose in anesthetized wild-type and v-/- mice in vivo. We measured absorption under basal and stimulated conditions (carbachol, vasoactive intestinal polypeptide, intralumen PGE(2)). Basal absorption and stimulated secretions were similar to those previously obtained in rats. There was no difference between wild-type and v-/- mice in animals with mixed genetic background or in pure C57BL6 mice. We conclude that this in vivo perfusion method is suitable for studying the absorption/secretion of electrolytes in the mouse intestine and that a lack of villin does not significantly alter basal and secretagogue-stimulated electrolyte movements across the epithelium of the mouse jejunum in vivo.
本研究旨在建立并验证一种用于测量小鼠小肠水和电解质吸收的单通道灌注方法。然后该方法被用于研究绒毛蛋白基因无效（v-/-）小鼠的肠道吸收。在体内对麻醉的野生型和v-/-小鼠的空肠进行单通道灌注，以测量水、Cl(-)、Na(+)、K(+)、HCO和葡萄糖的吸收。我们在基础条件和刺激条件（卡巴胆碱、血管活性肠肽、肠腔内PGE(2)）下测量吸收情况。基础吸收和刺激分泌与先前在大鼠中获得的结果相似。在具有混合遗传背景的动物或纯C57BL6小鼠中，野生型和v-/-小鼠之间没有差异。我们得出结论，这种体内灌注方法适用于研究小鼠肠道中电解质的吸收/分泌，并且缺乏绒毛蛋白不会显著改变体内小鼠空肠上皮细胞基础和促分泌剂刺激的电解质转运。
