Nicholas Kumudini U K G, Forney Charles F, Paulson Allan T
Agriculture and Agri-Food Canada, Atlantic Food and Horticulture Research Centre, 32 Main St., Kentville, N.S., Canada B4N IJ5.
Phytochem Anal. 2002 Jan-Feb;13(1):39-44. doi: 10.1002/pca.613.
A capillary gel electrophoretic (CGE) method for the quantitative analysis of RuBisCo in spinach leaves was developed. RuBisCo was resolved into large and small subunits in the presence of sodium dodecyl sulphate (SDS) by the CGE procedure which enabled the determination of the molecular weight of each unit accurately; the values so determined were in close agreement with those reported using other methods. Advantages of CGE over SDS-polyacrylamide gel electrophoresis and high-pressure gel filtration include decreased sample preparation and analysis time, superior resolution and greater sensitivity permitting reduced sample size and trace analysis. In addition, CGE provided precise quantification of RuBisCo and was demonstrated to be a viable alternative to other available methods of protein analysis.
开发了一种用于定量分析菠菜叶片中核酮糖-1,5-二磷酸羧化酶/加氧酶（RuBisCo）的毛细管凝胶电泳（CGE）方法。通过CGE程序，在十二烷基硫酸钠（SDS）存在下，RuBisCo被分解为大亚基和小亚基，这使得能够准确测定每个亚基的分子量；所测定的值与使用其他方法报道的值非常一致。与SDS-聚丙烯酰胺凝胶电泳和高压凝胶过滤相比，CGE的优点包括减少样品制备和分析时间、更高的分辨率和更高的灵敏度，从而允许减少样品量并进行痕量分析。此外，CGE提供了RuBisCo的精确定量，并被证明是其他现有蛋白质分析方法的可行替代方法。
