Ramirez E, Cartier L, Villota C, Fernandez J
Department of Virology, Public Health Institute of Chile, Av. Marathon 1000, Santiago, Chile.
Virus Res. 2002 Mar 20;84(1-2):135-49. doi: 10.1016/s0168-1702(02)00005-9.
Infection with Human T-Cell Lymphotropic Virus type I (HTLV-I) have been associated with the development of the HTLV-I associated myelopathy/tropical spastic paraparesis (HAM/TSP). Phylogenetic analyses of HTLV-I isolates have revealed that HTLV-I can be classified into three major groups: the Cosmopolitan, Central African and Melanesian. In the present study, we analyzed the tax, 5' ltr, gag, pol, and env sequences of proviruses of PBMC from ten HAM/TSP patients to investigate the phylogenetic characterization of HTLV-I in Chilean patients. HTLV-I provirus in PBMC from ten Chilean patients with HAM/TSP were amplified by PCR using primers of tax, 5' ltr, gag, pol, and env genes. Amplified products of the five genes were purified and nucleotide sequence was determined by the dideoxy termination procedure. DNA sequences were aligned with the CLUSTAL W program. The results of this study showed that the tax, 5' ltr, gag, pol, and env gene of the Chilean HTLV-I strains had a nucleotide homology ranged from 98.1 to 100%, 95 to 97%, 98.9 to 100%, 94 to 98%, and 94.2 to 98.5% respect to ATK-1 clone, respectively. According to molecular phylogeny with 5' ltr gene, the Chilean HTLV-I strains were grouped with each other suggesting one cluster included in Transcontinental subgroup.
感染人类嗜T细胞病毒I型（HTLV-I）与HTLV-I相关脊髓病/热带痉挛性截瘫（HAM/TSP）的发生有关。对HTLV-I分离株的系统发育分析表明，HTLV-I可分为三大类：世界性、中非和美拉尼西亚类。在本研究中，我们分析了10例HAM/TSP患者外周血单个核细胞（PBMC）前病毒的tax、5'长末端重复序列（5' ltr）、gag、pol和env序列，以研究智利患者中HTLV-I的系统发育特征。使用tax、5' ltr、gag、pol和env基因的引物，通过聚合酶链反应（PCR）扩增10例智利HAM/TSP患者PBMC中的HTLV-I前病毒。纯化五个基因的扩增产物，并通过双脱氧末端终止法测定核苷酸序列。DNA序列与CLUSTAL W程序进行比对。本研究结果表明，智利HTLV-I毒株的tax、5' ltr、gag、pol和env基因与ATK-1克隆相比，核苷酸同源性分别为98.1%至100%、95%至97%、98.9%至100%、94%至98%和94.2%至98.5%。根据5' ltr基因的分子系统发育分析，智利HTLV-I毒株相互聚类，表明在跨大陆亚组中有一个聚类。
