Yang G C, Morrison A B
Am J Clin Pathol. 1975 Nov;64(5):648-54. doi: 10.1093/ajcp/64.5.648.
Wide fields of tissue can be rapidly examined by electron microscopy by use of Formvar films for the support of ultrathin sections on slot grids. The intervention of the grid bars of conventional mesh grids is avoided, and a continuous micrograph of the specimen at scanning magnifications can be obtained. Enough material is sublimed from the section and the supporting film by deliberate exposure to the electron beam to permit one to obtain an image with good contrast. This method of examination, which takes in all about two hours, permits examination of an extensive area of tissue in relation both to its topography at low magnifications and to its ultrastructural detail, and accordingly adds to electron microscopy a dimension characteristic of the lower powers of the light microscope. It offers the histopathologist the option of using micrographs taken during the scanning survey of a tissue to detect regions that can be readily re-examined at high magnification in the same ultrathin section.
通过使用福尔马林膜在槽形网格上支撑超薄切片，电子显微镜可以快速检查大片组织。避免了传统网孔网格的网格条的干扰，并且可以获得扫描放大倍数下标本的连续显微照片。通过故意暴露于电子束，足够的材料从切片和支撑膜升华，从而使人们能够获得具有良好对比度的图像。这种检查方法总共大约需要两个小时，它能够检查组织的广泛区域，包括其低倍放大下的地形以及超微结构细节，因此为电子显微镜增添了光学显微镜低倍镜的特征维度。它为组织病理学家提供了一种选择，可以使用在组织扫描检查过程中拍摄的显微照片来检测那些可以在同一超薄切片中轻松进行高倍重新检查的区域。
