• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

一种用于检测牛胎儿三毛滴虫的改良聚合酶链反应检测方法。

An improved polymerase chain reaction assay for the detection of Tritrichomonas foetus in cattle.

作者信息

Nickel D Douglas, Olson Merle E, Schultz Gilbert A

机构信息

Department of Biochemistry and Molecular Biology, Health Sciences Centre, 3330 Hospital Drive NW, University of Calgary, Calgary, Alberta T2N 4N1.

出版信息

Can Vet J. 2002 Mar;43(3):213-6.

PMID:11901595
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339206/
Abstract

An improved polymerase chain reaction test has been developed to detect Tritrichomonas foetus, the causative agent of trichomoniasis in cattle. The test amplifies a region of the 5.8S ribosomal RNA gene of T. foetus, and it is simple, sensitive, and specific when compared with traditional methods to examine field samples.

摘要

一种改进的聚合酶链反应检测方法已被开发出来,用于检测牛毛滴虫病的病原体胎儿三毛滴虫。该检测方法扩增胎儿三毛滴虫5.8S核糖体RNA基因的一个区域,与检查现场样本的传统方法相比,它简单、灵敏且特异。

相似文献

1
An improved polymerase chain reaction assay for the detection of Tritrichomonas foetus in cattle.一种用于检测牛胎儿三毛滴虫的改良聚合酶链反应检测方法。
Can Vet J. 2002 Mar;43(3):213-6.
2
An improved molecular assay for Tritrichomonas foetus.一种改良的胎儿三毛滴虫分子检测方法。
Vet Parasitol. 2005 Jan 4;127(1):33-41. doi: 10.1016/j.vetpar.2004.08.018.
3
Improved detection of Tritrichomonas foetus in bovine diagnostic specimens using a novel probe-based real time PCR assay.使用基于新型探针的实时PCR检测方法改进牛诊断样本中胎儿三毛滴虫的检测。
Vet Parasitol. 2006 Nov 5;141(3-4):204-15. doi: 10.1016/j.vetpar.2006.06.012. Epub 2006 Jul 24.
4
Pooled polymerase chain reaction to detect Tritrichomonas foetus in beef bulls.用于检测公牛体内胎儿三毛滴虫的聚合酶链反应池
J Vet Diagn Invest. 2008 Jan;20(1):97-9. doi: 10.1177/104063870802000121.
5
Detection of Tritrichomonas foetus by polymerase chain reaction in cultured isolates, cervicovaginal mucus, and formalin-fixed tissues from infected heifers and fetuses.通过聚合酶链反应在培养的分离株、子宫颈阴道黏液以及来自感染小母牛和胎儿的福尔马林固定组织中检测胎儿三毛滴虫。
J Vet Diagn Invest. 2003 Nov;15(6):579-84. doi: 10.1177/104063870301500613.
6
PCR detection of Tritrichomonas foetus in preputial bull fluid without prior DNA isolation.在未事先进行DNA分离的情况下,对公牛包皮液中的胎儿三毛滴虫进行PCR检测。
Vet Parasitol. 2006 Mar 31;136(3-4):357-61. doi: 10.1016/j.vetpar.2005.11.016. Epub 2005 Dec 28.
7
PCR-based study of conserved and variable DNA sequences of Tritrichomonas foetus isolates from Saskatchewan, Canada.基于聚合酶链反应(PCR)对来自加拿大萨斯喀彻温省胎儿三毛滴虫分离株保守和可变DNA序列的研究。
J Clin Microbiol. 1995 May;33(5):1308-13. doi: 10.1128/jcm.33.5.1308-1313.1995.
8
Development of a bead-agglutination assay for rapid detection of Tritrichomonas foetus.用于快速检测胎儿三毛滴虫的珠凝集试验的开发。
Vet Parasitol. 2017 Aug 30;243:188-191. doi: 10.1016/j.vetpar.2017.06.025. Epub 2017 Jun 27.
9
Detection of bovine trichomoniasis with a specific DNA probe and PCR amplification system.使用特异性DNA探针和PCR扩增系统检测牛毛滴虫病
J Clin Microbiol. 1994 Jan;32(1):98-104. doi: 10.1128/jcm.32.1.98-104.1994.
10
Effects of bacterial contamination of media on the diagnosis of Tritrichomonas foetus by culture and real-time PCR.培养基细菌污染对通过培养和实时荧光定量PCR诊断胎儿三毛滴虫的影响。
Vet Parasitol. 2015 Mar 15;208(3-4):143-9. doi: 10.1016/j.vetpar.2015.01.006. Epub 2015 Jan 19.

引用本文的文献

1
Bovine trichomoniasis: A hidden threat to reproductive efficiency.牛毛滴虫病:对繁殖效率的潜在威胁。
Open Vet J. 2024 Nov;14(11):2722-2730. doi: 10.5455/OVJ.2024.v14.i11.1. Epub 2024 Nov 30.
2
Molecular detection of Tritrichomonas foetus in bovine samples: a novel real-time polymerase chain reaction (PCR) assay targeting EF1-alpha-Tf1 and a comparative study of published PCR techniques.牛样品中胎儿三毛滴虫的分子检测:一种针对 EF1-α-Tf1 的新型实时聚合酶链反应(PCR)检测方法和已发表 PCR 技术的比较研究。
Parasitol Res. 2022 Jun;121(6):1725-1733. doi: 10.1007/s00436-022-07487-7. Epub 2022 Mar 29.
3
Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): Trichomonosis.在《动物卫生法》(欧盟第2016/429号条例)框架内对动物疾病进行列名和分类的评估:毛滴虫病
EFSA J. 2017 Oct 12;15(10):e04992. doi: 10.2903/j.efsa.2017.4992. eCollection 2017 Oct.

本文引用的文献

1
Saskatchewan. Trichomonas fetus in bulls in Saskatchewan.萨斯喀彻温省。萨斯喀彻温省公牛体内的胎儿三毛滴虫。
Can Vet J. 1993 Sep;34(9):571-2.
2
The establishment of various trichomonads of animals and man in axenic cultures.动物和人类各种毛滴虫在无菌培养中的建立。
J Parasitol. 1957 Aug;43(4):488-90.
3
Prevalence of Tritrichomonas fetus in a bull population and effect on production in a large cow-calf enterprise.公牛群体中胎儿三毛滴虫的流行情况及其对大型母牛-犊牛企业生产的影响。
J Am Vet Med Assoc. 1999 Apr 1;214(7):1051-5.
4
Comparative sequence analysis of 5.8S rRNA genes and internal transcribed spacer (ITS) regions of trichomonadid protozoa.毛滴虫原生动物5.8S rRNA基因和内部转录间隔区(ITS)区域的比较序列分析
Parasitology. 1997 Aug;115 ( Pt 2):111-9. doi: 10.1017/s0031182097001212.
5
Detection of Tritrichomonas foetus by PCR and DNA enzyme immunoassay based on rRNA gene unit sequences.基于rRNA基因单位序列,通过聚合酶链反应(PCR)和DNA酶免疫测定法检测胎儿三毛滴虫。
J Clin Microbiol. 1998 Feb;36(2):513-9. doi: 10.1128/JCM.36.2.513-519.1998.
6
Detection of bovine trichomoniasis with a specific DNA probe and PCR amplification system.使用特异性DNA探针和PCR扩增系统检测牛毛滴虫病
J Clin Microbiol. 1994 Jan;32(1):98-104. doi: 10.1128/jcm.32.1.98-104.1994.
7
PCR-based study of conserved and variable DNA sequences of Tritrichomonas foetus isolates from Saskatchewan, Canada.基于聚合酶链反应(PCR)对来自加拿大萨斯喀彻温省胎儿三毛滴虫分离株保守和可变DNA序列的研究。
J Clin Microbiol. 1995 May;33(5):1308-13. doi: 10.1128/jcm.33.5.1308-1313.1995.
8
A simple salting out procedure for extracting DNA from human nucleated cells.一种从人有核细胞中提取DNA的简单盐析方法。
Nucleic Acids Res. 1988 Feb 11;16(3):1215. doi: 10.1093/nar/16.3.1215.
9
Prevalence of trichomoniasis among California beef herds.加利福尼亚肉牛群中滴虫病的患病率。
J Am Vet Med Assoc. 1990 May 15;196(10):1590-3.
10
Basic local alignment search tool.基本局部比对搜索工具
J Mol Biol. 1990 Oct 5;215(3):403-10. doi: 10.1016/S0022-2836(05)80360-2.