Felleisen R S
Institute of Parasitology, University of Bern, Switzerland.
Parasitology. 1997 Aug;115 ( Pt 2):111-9. doi: 10.1017/s0031182097001212.
The taxonomic situation in the genus Tritrichomonas is the subject of controversial discussion: potentially T. foetus and T. suis, the tritrichomonads from cattle and swine, respectively, could belong to the same species. In order to shed some light on this question, a molecular biological analysis was performed. The 5.8S rRNA gene and the flanking internal transcribed spacer regions (ITS1 and ITS2) of 12 different isolates of 3 Tritrichomonas species T. foetus, T. suis and T. mobilensis were enzymatically amplified by PCR and subcloned. Also, the corresponding regions of the trichomonads Trichomonas vaginalis, T. tenax, T. gallinae and Pentatrichomonas hominis were included in this study. Sequence analysis of cloned fragments was used to compare the parasite isolates. The genus Tritrichomonas exhibited an extremely high degree of homogeneity. All T. foetus and T. suis isolates had identical sequences, and only 1 substitution was found in the ITS2 region of T. mobilensis. In contrast, the genus Trichomonas shared more diversity. The results obtained in this study support a possible future revision of the taxonomic classification of tritrichomonads.
潜在地，胎儿三毛滴虫和猪三毛滴虫，分别来自牛和猪的三毛滴虫，可能属于同一物种。为了阐明这个问题，进行了分子生物学分析。通过PCR酶促扩增并亚克隆了胎儿三毛滴虫、猪三毛滴虫和活动三毛滴虫这3种三毛滴虫属的12个不同分离株的5.8S rRNA基因及侧翼内部转录间隔区（ITS1和ITS2）。此外，阴道毛滴虫、口腔毛滴虫、鸡毛滴虫和人五毛滴虫的相应区域也包含在本研究中。利用克隆片段的序列分析来比较寄生虫分离株。三毛滴虫属表现出极高的同源性。所有胎儿三毛滴虫和猪三毛滴虫分离株具有相同的序列，并且在活动三毛滴虫的ITS2区域仅发现1个替换。相比之下，毛滴虫属具有更多样性。本研究获得的结果支持未来可能对三毛滴虫分类进行修订。
