Zheng Xiu-Fen, Cheng Jun-Feng, Ye Jian, Zhu Shao-Jian
Institute of Forensic Science, Beijing 100038, China.
Yi Chuan Xue Bao. 2002 Feb;29(2):95-100.
We have obtained the alleles and haplotype distribution of 100 unrelated males from the Chinese Han and Zhuang populations at the four Y chromosome-specific loci A10, C4, A7.1, A7.2 by fluorescent primers and with 377 DNA sequencer. We observed 7, 6, 6, 6 alleles in loci A10, C4, A7.1, A7.2 respectively, the gene diversity (GD) values are 0.7776/0.629, 0.773/0.732, 0.5978/0.7272, 0.6664/0.6458 (Zhuang/Han). 114 haplotypes were found in 200 males, haplotype diversity (HD) values are 0.9786, 0.9772 (Zhuang/Han). We have confirmed the core repeats and its repeating number of these alleles by sequencing. A tetraplex PCR system consisting of the A10, C4, A7.1 and A7.2 loci was set up and it is of good specificity. We investigated the validation at these four Y-STRs loci in forensic application such as the male specificity, genetic stability and the sensitivity. In conclusion, the four Y-STRs loci are very suitable to forensic medicine analysis and paternity test.
我们使用荧光引物和377型DNA测序仪，获得了中国汉族和壮族100名无关男性在四个Y染色体特异性位点A10、C4、A7.1、A7.2的等位基因和单倍型分布。我们分别在A10、C4、A7.1、A7.2位点观察到7、6、6、6个等位基因，基因多样性（GD）值分别为0.7776/0.629、0.773/0.732、0.5978/0.7272、0.6664/0.6458（壮族/汉族）。在200名男性中发现了114种单倍型，单倍型多样性（HD）值分别为0.9786、0.9772（壮族/汉族）。我们通过测序确认了这些等位基因的核心重复序列及其重复次数。建立了一个由A10、C4、A7.1和A7.2位点组成的四重PCR系统，其特异性良好。我们研究了这四个Y-STR位点在法医应用中的有效性，如男性特异性、遗传稳定性和灵敏度。总之，这四个Y-STR位点非常适合法医学分析和亲子鉴定。
