[潮汕人群12个Y染色体短串联重复序列基因座的遗传多态性分析]

[An analysis for genetic polymorphism of twelve Y short tandem repeat loci in Chaoshan populations].

作者信息

Liu Jing-wen, Hu Sheng-ping, Li Biao

机构信息

Center for Molecular Biology, Medical College, Shantou University, Shantou, Guangdong, 515031 PR China.

出版信息

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2006 Jun;23(3):352-4.

PMID:16767684

Abstract

OBJECTIVE

To study the genetic polymorphism of twelve short tandem repeat loci of Y chromosome in Chaoshan Han populations.

METHODS

Twelve Y short tandem repeat loci, of which the template DNAs were extracted from blood samples of 121 unrelated Chaoshan Han individuals, were amplified by using the PowerPlex Y system. The PCR products were genotyped with ABI PRISM 3100 genetic analyzer.

RESULTS

From the loci DYS19, DYS437, DYS389I, DYS389II, DYS438, DYS439, DYS393, DYS391, DYS390, and DYS392, 4-7 alleles were checked out; from the loci DYS385, 35 alleles were observed. The overall observed haplotype diversity reached 0.9976. DYS391 was the locus with the lowest gene diversity (0.4445), while DYS385 had the highest gene diversity (0.9525).

CONCLUSION

The 12 Y-STR loci are highly polymorphic and suitable to forensic casework, population genetics and anthropology studies.

摘要

目的

研究潮汕汉族人群Y染色体12个短串联重复序列位点的基因多态性。

方法

采用PowerPlex Y系统对121名无血缘关系的潮汕汉族个体血样提取模板DNA，扩增12个Y染色体短串联重复序列位点。PCR产物用ABI PRISM 3100基因分析仪进行基因分型。

结果

在DYS19、DYS437、DYS389I、DYS389II、DYS438、DYS439、DYS393、DYS391、DYS390和DYS392位点检出4 - 7个等位基因；在DYS385位点观察到35个等位基因。总体观察单倍型多样性达到0.9976。DYS391是基因多样性最低的位点（0.4445），而DYS385基因多样性最高（0.9525）。