Strenkoski L F, Freake R, Wiemeri C A
Am J Med Technol. 1975 Oct;41(10):353-9.
A novel, convenient, multiple test system for the detection of bacteriuria is described and evaluated. The system consists of a small plastic strip with three dry reagent areas. One rapidly detects the presence of nitrite in urine and the remaining two quantitate total and gram-negative bacterial urine population after 18 to 24 hours of incubation. Quantitative accuracy was evaluated in two blind studies. In the first, known concentrations of 18 different organisms were compared and in the second, unknown concentrations of 218 randomly collected urine specimens were quantitated by both the calibrated loop streak method the new system. Overall correlation was 96 per cent for growth reagent areas. The nitrite area detected 84 per cent of the urines positive for bacteriuria. The advantages of the system over conventional bacteriuria detection systems are described.
本文描述并评估了一种用于检测菌尿症的新型便捷多重检测系统。该系统由一条带有三个干试剂区的小塑料条组成。一个区域可快速检测尿液中亚硝酸盐的存在,另外两个区域在培养18至24小时后对尿液中的总细菌数和革兰氏阴性细菌数进行定量。在两项盲法研究中评估了定量准确性。第一项研究比较了18种不同生物体的已知浓度,第二项研究通过校准环划线法和新系统对218份随机收集的尿液标本的未知浓度进行了定量。生长试剂区的总体相关性为96%。亚硝酸盐区域检测出84%菌尿阳性的尿液。文中还描述了该系统相对于传统菌尿检测系统的优势。