Hérault Yann, Kmita Marie, Sawaya Chadi C, Duboule Denis
Department of Zoology and Animal Biology, University of Geneva, Switzerland.
Int J Dev Biol. 2002 Jan;46(1):185-91.
In mice, the loxP/Cre recombinase-dependent system of recombination offers powerful possibilities for engineering genetic configurations of interest. This system can also be advantageously used for conditional mutagenesis in vivo, whenever such an approach is required due to deleterious effects of either one mutation, or a combination thereof. Here, we report on the production of an allelic series of insertions of a Hoxd11/Cre fusion transgene at different positions within the HoxD complex, in order to produce the CRE recombinase with a 'Hox profile' progressively more extended. We used the R26R (R26R) reporter mouse line to functionally assess the distribution and efficiency of the CRE enzyme and discuss the usefulness of these various lines as deleter strains.
在小鼠中，loxP/Cre重组酶依赖性重组系统为构建感兴趣的基因构型提供了强大的可能性。只要由于单个突变或其组合的有害影响而需要这种方法，该系统也可有利地用于体内条件性诱变。在此，我们报告了在HoxD复合体的不同位置产生Hoxd11/Cre融合转基因插入的等位基因系列，以便产生具有逐渐更广泛“同源框表达模式”的CRE重组酶。我们使用R26R报告小鼠品系从功能上评估CRE酶的分布和效率，并讨论这些不同品系作为缺失菌株的实用性。
