Pritchard J B, O'Connor N, Oliver J M, Berlin R D
Am J Physiol. 1975 Oct;229(4):967-72. doi: 10.1152/ajplegacy.1975.229.4.967.
We have analyzed for purine compounds entering and leaving the liver in lightly anesthetized rabbits and rats and for the export of utilizable purine from liver perfused with oxypurine. The in vivo results indicate that roughly 80% of hypoxanthine, xanthine, and urate is removed in a single passage of blood through liver. Conversely, the adenosine concentration of hepatic venous blood is increased 10-fold over portal or arterial levels. When the liver is isolated and perfused with hypoxanthine there is significant release of adenosine, whether measured quantitatively by microbiological assay or qualitatively by analysis of the radioactive purines released from liver that has been prelabeled with [14C]hypoxanthine. These results provide direct evidence for the clearance of hydroxylated purines and the release of utilizable adenine derivatives by liver.
我们分析了轻度麻醉的兔和大鼠体内进出肝脏的嘌呤化合物，以及用氧嘌呤灌注肝脏时可利用嘌呤的输出情况。体内实验结果表明，次黄嘌呤、黄嘌呤和尿酸在血液单次流经肝脏时大约80%被清除。相反，肝静脉血中的腺苷浓度比门静脉或动脉血中的浓度增加了10倍。当肝脏被分离并用次黄嘌呤灌注时，无论通过微生物学测定法定量测量，还是通过分析预先用[14C]次黄嘌呤标记的肝脏释放的放射性嘌呤定性测量，都会有大量腺苷释放。这些结果为肝脏清除羟基化嘌呤和释放可利用的腺嘌呤衍生物提供了直接证据。
