Landsberger Martin, Lorković Zdravko J, Oelmüller Ralf
Institut für Allgemeine Botanik der Friedrich-Schiller-Universität, Lehrstuhl für Pflanzenphysiologie, Jena, Germany.
Plant Mol Biol. 2002 Mar;48(4):413-21. doi: 10.1023/a:1014089531125.
We report the isolation and characterization of genes from the higher plants Arabidopsis, spinach and tobacco which code for nucleus-localized RNA-binding proteins. Common features of these polypeptides are glycine/arginine-rich regions with several RGG repeats at their N- and C-termini, which are sufficient for RNA binding in northwestern assays. All polypeptides analysed contain two basic bipartite nuclear localization signals and translational fusions harbouring these regions with the beta-glucuronidase gene direct the fusion proteins into the nucleus. Nuclear localization was confirmed by cellular fractionation with a polyclonal antiserum raised against the over-expressed tobacco protein NtRGG1p. Two or three copies of related RGG genes appear to be present in the analysed organisms and the expression of some of them is regulated: a tobacco gene is light-regulated and a spinach gene is preferentially expressed in roots. Possible biological functions of this class of RNA-binding proteins as well as structure/function relationships related to the modular structure are discussed.
我们报道了从高等植物拟南芥、菠菜和烟草中分离并鉴定出编码定位于细胞核的RNA结合蛋白的基因。这些多肽的共同特征是在其N端和C端富含甘氨酸/精氨酸区域，带有几个RGG重复序列，这在蛋白质印迹检测中足以实现RNA结合。所有分析的多肽都含有两个基本的双分型核定位信号，携带这些区域与β-葡萄糖醛酸酶基因的翻译融合体将融合蛋白导向细胞核。通过用针对过表达的烟草蛋白NtRGG1p产生的多克隆抗血清进行细胞分级分离，证实了核定位。在所分析的生物体中似乎存在两到三个相关的RGG基因拷贝，其中一些基因的表达受到调控：一个烟草基因受光调控，一个菠菜基因在根中优先表达。讨论了这类RNA结合蛋白可能的生物学功能以及与模块化结构相关的结构/功能关系。
