Leung Anskar Y H, Chan Maggie, Tang Sydney C W, Liang Raymond, Kwong Y L
University Department of Medicine, Professorial Block, Queen Mary Hospital, Pokfulam Road, Hong Kong.
J Virol Methods. 2002 May;103(1):51-6. doi: 10.1016/s0166-0934(01)00447-5.
Polyoma BK virus (BKV) remains dormant in the urinary tract and circulating leucocytes and becomes reactivated during immunosuppression. BK viruria is prevalent in renal allograft recipients and BK viremia may be related to nephropathy and allograft rejection. How BK viruria and viremia are related in renal allograft patients is undefined. In this study, BKV copies in paired urine and serum samples of renal allograft recipients were measured by a real time quantitative polymerase chain reaction (Q-PCR) to test the hypothesis that their quantitative relationship might help to delineate viral reactivation patterns in these tissues. Urine and plasma samples from 44 renal allograft recipients with stable graft function were collected during outpatient follow-up and the genome copies of BKV were determined by Q-PCR. All patients showed quantifiable viremia and two groups of patients were identified: one group of patients (n=35) showed low viral load (median: 270/ml, range: 108-1000/ml) and the other group (n=9) with high viral load (median: 5x10(4)/ml, range: 2x10(4)-6x10(4)/ml). The corresponding median levels of viruria were 2000 and 900 ml. BK viremia and viruria were not related quantitatively. BK viremia/viruria were also not related to age, immunosuppression, time and source of renal grafts and serum creatinine levels. The absence of a quantitative relationship between BK viremia and viruria may reflect independent BKV reactivation in different tissues during immunosuppression.
多瘤BK病毒（BKV）在尿路和循环白细胞中保持潜伏状态，并在免疫抑制期间重新激活。BK病毒尿症在肾移植受者中很常见，BK病毒血症可能与肾病和移植肾排斥反应有关。在肾移植患者中，BK病毒尿症和病毒血症之间的关系尚不清楚。在本研究中，通过实时定量聚合酶链反应（Q-PCR）测量肾移植受者配对的尿液和血清样本中的BKV拷贝数，以检验其定量关系可能有助于描绘这些组织中病毒重新激活模式的假设。在门诊随访期间收集了44例移植肾功能稳定的肾移植受者的尿液和血浆样本，并通过Q-PCR测定BKV的基因组拷贝数。所有患者均表现出可量化的病毒血症，并确定了两组患者：一组患者（n = 35）病毒载量低（中位数：270/ml，范围：108 - 1000/ml），另一组（n = 9）病毒载量高（中位数：5×10⁴/ml，范围：2×10⁴ - 6×10⁴/ml）。相应的病毒尿症中位数水平分别为2000和900/ml。BK病毒血症和病毒尿症在数量上没有相关性。BK病毒血症/病毒尿症也与年龄、免疫抑制、肾移植的时间和来源以及血清肌酐水平无关。BK病毒血症和病毒尿症之间缺乏定量关系可能反映了免疫抑制期间不同组织中BKV的独立重新激活。
