Vázquez Susana, Guzmán María Guadalupe, Guillen Gerardo, Chinea Glay, Pérez Ana Beatriz, Pupo Maritza, Rodriguez Rosmary, Reyes Osvaldo, Garay Hilda Elisa, Delgado Iselys, García Gissel, Alvarez Mayling
Virology Department, "Pedro Kouri" Tropical Medicine Institute, Autopista Novia del Mediodia, km 6, P.O. Box Marianao 13, Havana, Cuba.
Vaccine. 2002 Mar 15;20(13-14):1823-30. doi: 10.1016/s0264-410x(01)00515-1.
The immunological activities of five synthetic peptides of the prM protein of dengue-2 (DEN-2) virus containing B cell epitopes were evaluated in BALB/c mice. Two peptides elicited neutralizing antibodies against all four DEN serotypes. Virus-specific proliferative responses were demonstrated in mice immunized with four of the five peptides, demonstrating the presence of T cell epitopes. Mice immunized with three of the five peptides conjugated with bovine albumin showed statistically significant levels (P<0.05) of protection when challenged with DEN-2 virus. These results could constitute the basis for the establishment of the role of DEN virus pre and M antigens in the development of anti-flaviviral vaccines.
在BALB/c小鼠中评估了登革2型（DEN-2）病毒prM蛋白的五个含B细胞表位的合成肽的免疫活性。两种肽引发了针对所有四种登革血清型的中和抗体。在用五个肽中的四个免疫的小鼠中显示出病毒特异性增殖反应，证明了T细胞表位的存在。用与牛白蛋白偶联的五个肽中的三个免疫的小鼠在用DEN-2病毒攻击时表现出具有统计学意义（P<0.05）的保护水平。这些结果可为确定DEN病毒前体和M抗原在抗黄病毒疫苗开发中的作用奠定基础。
