Ohtori S, Yamamoto T, Ino H, Hanaoka E, Shinbo J, Ozaki T, Takada N, Nakamura Y, Chiba T, Nakagawara A, Sakiyama S, Sakashita Y, Takahashi K, Tanaka K, Yamagata M, Yamazaki M, Shimizu S, Moriya H
Department of Orthopaedic Surgery, School of Medicine, Chiba University, Japan.
Neuroscience. 2002;110(3):579-86. doi: 10.1016/s0306-4522(01)00590-5.
Differential screening-selected gene aberrative in neuroblastoma (DAN) belongs to a novel gene family that includes the Xenopus head-inducing factor, Cerberus and the dorsalizing factor, Gremlin. It has been suggested that members of this family control diverse processes in growth, development and the cell cycle.Here, we demonstrate that the DAN protein is produced in the small neurons of the dorsal root ganglion and is transported to the nerve terminals in the spinal dorsal horn in adult rats. Furthermore, intrathecal injection of an antibody to the DAN protein suppressed inflammatory pain caused by the introduction of complete Freund's adjuvant or carrageenan into the rat hindpaw. The amount of mRNA for DAN in dorsal root ganglion neurons and of its expressed protein in the spinal dorsal horn were both increased in inflammatory models.Together, these data suggest that the DAN protein may be a novel neuromodulator in primary nociceptive nerve fibers.
神经母细胞瘤差异筛选选择的异常基因（DAN）属于一个新的基因家族，该家族包括非洲爪蟾头部诱导因子、Cerberus和背化因子Gremlin。有人提出，这个家族的成员控制着生长、发育和细胞周期中的多种过程。在此，我们证明DAN蛋白在成年大鼠背根神经节的小神经元中产生，并被运输到脊髓背角的神经末梢。此外，鞘内注射抗DAN蛋白抗体可抑制因向大鼠后爪注射完全弗氏佐剂或角叉菜胶而引起的炎性疼痛。在炎性模型中，背根神经节神经元中DAN的mRNA量及其在脊髓背角中表达的蛋白量均增加。这些数据共同表明，DAN蛋白可能是初级伤害性神经纤维中的一种新型神经调节剂。
