Rozenski Jef, McCloskey James A
Rega Institute for Medical Research, Katholieke Universiteit Leuven, Belgium.
J Am Soc Mass Spectrom. 2002 Mar;13(3):200-3. doi: 10.1016/S1044-0305(01)00354-3.
Mass spectra of oligonucleotides derived from collision-induced dissociation following electrospray ionization provide an effective means of sequence determination, at the 20-mer level and below. An interactive, stand-alone computer program, Simple Oligonucleotide Sequencer (SOS) has been developed for rapid oligonucleotide sequencing from mass spectra, under user control on a residue by residue basis. Modifications can be defined in any combination for the base, sugar or backbone. Sequence ladders can be independently constructed in both the 5' --> 3' directions and 3' --> 5' directions, and graphically compared for homology and overlap. A particular advantage of this method is the ability to easily erase and rebuild alternate subsequences. The program can be used for ab initio sequencing of modified or unmodified oligonucleotides, for rapid verification of sequence, and in studies of fragmentation processes of model oligonucleotide derivatives.
电喷雾电离后碰撞诱导解离产生的寡核苷酸质谱，为20聚体及以下水平的序列测定提供了一种有效的方法。已经开发了一个交互式独立计算机程序，即简易寡核苷酸测序仪（SOS），用于在用户控制下逐个残基地从质谱中快速进行寡核苷酸测序。碱基、糖或主链的修饰可以以任何组合定义。序列梯可以在5'→3'方向和3'→5'方向上独立构建，并以图形方式比较同源性和重叠情况。该方法的一个特别优点是能够轻松擦除并重建替代子序列。该程序可用于修饰或未修饰寡核苷酸的从头测序、序列的快速验证以及模型寡核苷酸衍生物的片段化过程研究。
