Wein Samuel
Center for Bioinformatics Tübingen, University of Tübingen, Tübingen, Germany.
Methods Mol Biol. 2023;2624:225-239. doi: 10.1007/978-1-0716-2962-8_15.
Mass spectrometry is an ideal method for the discovery and characterization of modified RNAs. Unlike other traditional sequencing methods, mass spectrometry can identify and localize multiple types of modifications in tandem. One of the traditional hurdles to using this powerful technique has been a paucity of software to interpret the complicated data produced by these experiments. Here I describe how to use the NucleicAcidSearchEngine (NASE), a component of OpenMS as well as best practices for acquiring RNA data, and potential pitfalls in the analysis process.
质谱分析法是发现和鉴定修饰RNA的理想方法。与其他传统测序方法不同，质谱分析法可以串联识别和定位多种类型的修饰。使用这种强大技术的传统障碍之一是缺乏用于解释这些实验产生的复杂数据的软件。在此，我将描述如何使用核酸搜索引擎（NASE），它是OpenMS的一个组件，以及获取RNA数据的最佳实践，还有分析过程中可能存在的陷阱。
