Bushek David, Dungan Christopher F, Lewitus Alan J
University of South Carolina, Belle W. Baruch Institute for Marine Biology and Coastal Research, Baruch Marine Field Laboratory, Georgetown 29442, USA.
J Eukaryot Microbiol. 2002 Jan-Feb;49(1):11-6. doi: 10.1111/j.1550-7408.2002.tb00333.x.
The protozoan oyster pathogen Perkinsus marinus is classified in the phylum Apicomplexa, although molecular-genetic and ultrastructural evidence increasingly concur on its closer phylogenetic relationship with the dinoflagellates. To test for evidence of serological epitopes common to P. marinus and dinoflagellates, we probed 19 free-living and 8 parasitic dinoflagellate, or dinoflagellate-like, species for cross-reactivity with polyclonal antibodies to P. marinus. Three of 19 free-living dinoflagellates (16%), and 7 of 8 parasitic dinoflagellates (88%) were labeled by anti-P. marinus antibodies. In reciprocal immunoassays using polyclonal antibodies to the Hematodinium sp. dinoflagellate parasite of Norway lobsters, Nephrops norvegicus, P. marinus and the same 7 parasitic dinoflagellates labeled by anti-P. marinus antibodies, were again labeled. The dinoflagellate-like parasite of prawns Pandalus platyceros was not labeled by either antibody reagent. These reciprocal results confirm the presence of shared antibody-binding epitopes on cells of P. marinus and several dinoflagellates. The apparent widespread serological affinity between P. marinus and the parasitic dinoflagellates suggests a closer phylogenetic link to the syndinean dinoflagellate lineage. The consistent failure of the dinoflagellate-like prawn parasite to bind either antibody reagent shows that this parasite is serologically distinct from both P. marinus and Hematodinium-species parasitic dinoflagellates.
原生动物牡蛎病原体——海洋派琴虫（Perkinsus marinus）被归类于顶复门（Apicomplexa），尽管分子遗传学和超微结构证据越来越多地表明它与甲藻在系统发育上关系更为密切。为了检测海洋派琴虫和甲藻共有的血清学表位的证据，我们用针对海洋派琴虫的多克隆抗体检测了19种自由生活的和8种寄生性甲藻或类甲藻物种的交叉反应性。19种自由生活的甲藻中有3种（16%），8种寄生性甲藻中有7种（88%）被抗海洋派琴虫抗体标记。在使用针对挪威龙虾（Nephrops norvegicus）的血红裸藻（Hematodinium sp.）甲藻寄生虫的多克隆抗体进行的双向免疫测定中，海洋派琴虫以及被抗海洋派琴虫抗体标记的同样7种寄生性甲藻再次被标记。对虾（Pandalus platyceros）的类甲藻寄生虫未被任何一种抗体试剂标记。这些双向结果证实了海洋派琴虫和几种甲藻细胞上存在共同的抗体结合表位。海洋派琴虫与寄生性甲藻之间明显广泛的血清学亲和力表明它与共生甲藻谱系在系统发育上有更密切的联系。类甲藻对虾寄生虫始终无法与任何一种抗体试剂结合，这表明该寄生虫在血清学上与海洋派琴虫和血红裸藻属寄生性甲藻均不同。
