Müller Annette M, Skrzynski Carmen, Nesslinger Maya, Skipka Guido, Müller Klaus-Michael
Institute of Pathology, Berufsgenossenschaftliche Kliniken Bergmannsheil-Universitätsklinik, Bürkle-de-la-Camp-Platz 1, D-44789 Bochum, Germany.
Exp Gerontol. 2002 May;37(5):713-9. doi: 10.1016/s0531-5565(02)00010-4.
The importance of endothelial senescence as a pathogenetic factor in age-related vascular alterations has almost exclusively been studied in vitro. However, the in vitro-findings have rarely been compared with histomorphological changes in aging human tissue or in age-related degenerative diseases. Therefore, we compared the expression of the endothelial marker CD34 and the procoagulant protein von Willebrand factor (vWf) in lung endothelium by conventional immunohistochemistry and confocal laser scan microscopy (CLSM), taking the age of the patients into consideration. The staining reactions were statistically analysed by covariance analysis. With age the endothelial staining intensity of CD34 increased in arteries and veins, but decreased in arterioles, capillaries and venules. For vWf, on the contrary, the endothelial staining intensity increased with age in all types of vessels. CLSM confirmed a mosaic staining pattern. This study demonstrates age-associated phenotypical alterations of CD34 and vWf. Whether the down-regulation of CD34 correlates with an age-associated reduction of the angiogenetic properties of EC or an age-related over-expression of vWf as a relevant cofactor for the raised coagulatory activity and the increase in thrombotic diseases resp coronary heart disease in older patients, remains subject to debate.
内皮细胞衰老作为年龄相关性血管改变的致病因素，其重要性几乎完全是在体外进行研究的。然而，体外研究结果很少与衰老人体组织或年龄相关性退行性疾病中的组织形态学变化进行比较。因此，我们通过传统免疫组织化学和共聚焦激光扫描显微镜（CLSM），考虑患者年龄，比较了肺内皮中内皮标志物CD34和促凝蛋白血管性血友病因子（vWf）的表达。通过协方差分析对染色反应进行统计学分析。随着年龄增长，动脉和静脉中CD34的内皮染色强度增加，但在小动脉、毛细血管和小静脉中降低。相反，对于vWf，所有类型血管中的内皮染色强度均随年龄增长而增加。CLSM证实了镶嵌染色模式。本研究证明了CD34和vWf与年龄相关的表型改变。CD34的下调是否与内皮细胞血管生成特性的年龄相关性降低相关，或者vWf作为老年患者凝血活性升高和血栓性疾病及冠心病增加的相关辅助因子的年龄相关性过表达，仍有待讨论。
