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[利用极高频辐射从土壤中分离放线菌]

[Isolation of actinomycetes from soil using extremely high frequency radiation].

作者信息

Li Iu V, Terekhova L P, Gapochka M G

机构信息

Research Institute of New Antibiotics, Russian Academy of Medical Sciences, ul. Bol'shaya Pirogovskaya 11, Moscow, 119867 Russia.

出版信息

Mikrobiologiia. 2002 Jan-Feb;71(1):119-22.

Abstract

A new method employing extremely high frequencies (EHFs) is proposed for the selective isolation of actinomycetes from soil. The pretreatment of soil suspensions with EHF wavelengths of 5.6 and 7.1 mm led to a nonselective isolation of actinomycetes. At the same time, the irradiation of soil suspensions within wavelength bands of 3.8-5.8 and 8-11.5 mm considerably augmented the total number of isolated actinomycetes and increased the fraction of the isolated rare genera by 2 and 7 times, respectively. The rare actinomycete genera were represented by Actinomadura, Microtetraspora, Nonomuraea, Micromonospora, Amycolatopsis, Pseudonocardia, Saccharotrix, and Streptosporangium.

摘要

提出了一种采用极高频(EHF)从土壤中选择性分离放线菌的新方法。用波长为5.6和7.1毫米的极高频对土壤悬浮液进行预处理,可实现放线菌的非选择性分离。同时,在3.8 - 5.8毫米和8 - 11.5毫米波段对土壤悬浮液进行辐照,可使分离出的放线菌总数显著增加,且使分离出的稀有属的比例分别提高2倍和7倍。稀有放线菌属包括马杜拉放线菌属、小四孢菌属、野野村菌属、小单孢菌属、拟无枝酸菌属、假诺卡氏菌属、糖多孢菌属和链孢囊菌属。

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