Li Iu V, Terekhova L P, Alferova I V, Galatenko O A, Gapochka M G
Research Institute of New Antibiotics, Russian Academy of Medical Sciences, ul. Bol'shaya Pirogovskaya 11, Moscow, 119867 Russia.
Mikrobiologiia. 2003 Jan-Feb;72(1):131-5.
A new method employing succession analysis and extremely high-frequency (EHF) irradiation is proposed for the selective isolation of actinomycetes from soil. Total actinomycetes were efficiently isolated from soil suspensions irradiated in the wavelength band 4.6-5.8 mm on the 14th and 45th days of succession initiated by soil wetting and from soil suspensions irradiated in the wavelength band 8-11.5 mm on the 7th day of succession. The rare actinomycete genera Actinomadura, Micromonospora, Nonomuraea, Microbispora, Amycolatopsis, Pseudonocardia, Saccharothrix, Streptosporangium, Actinosynnema, Nocardioides, and Saccharopolyspora were isolated by either of the two approaches (succession analysis and EHF irradiation); however, the range of isolated rare actinomycetes was considerably wider when the combination of the two approaches was used. For instance, actinomycetes of the rare genera Actinocorallia, Promicromonospora, Actinoplanes, and Kibdelosporangium were isolated only when EHF irradiation was employed at the early stages of succession.
提出了一种采用演替分析和极高频（EHF）辐射从土壤中选择性分离放线菌的新方法。在土壤湿润引发演替的第14天和第45天，从波长范围为4.6 - 5.8毫米的土壤悬浮液中，以及在演替第7天从波长范围为8 - 11.5毫米的土壤悬浮液中，能有效分离出总放线菌。通过两种方法（演替分析和EHF辐射）中的任何一种，均可分离出稀有放线菌属马杜拉放线菌属、小单孢菌属、野野村菌属、小双孢菌属、拟无枝酸菌属、假诺卡氏菌属、糖丝菌属、链孢囊菌属、放线共生菌属、诺卡氏菌属和糖多孢菌属；然而，当两种方法结合使用时，分离出的稀有放线菌范围要广泛得多。例如，稀有属的放线珊瑚菌属、原小单孢菌属、游动放线菌属和栖土孢囊菌属的放线菌，仅在演替早期采用EHF辐射时才能分离得到。
