Bulina T I, Terekhova L P, Tiurin M V
Research Institute of New Antibiotics, Russian Academy of Medical Sciences, Moscow, Russia.
Mikrobiologiia. 1998 Jul-Aug;67(4):556-60.
A new method was elaborated for the selective isolation of actinomycetes from soil, which involves the pretreatment of soil samples with electric pulses. The treatment of soil with 3-ms electric pulses at a field intensity of 12 kV/cm2 resulted in a significant (threefold on average) increase in the proportion of representatives of rare actinomycetes isolated. This treatment regime was found to be most efficient for the selective isolation of representatives of the genera Actinoplanes, Micromonospora, Actinomadura, and Microtetraspora. The proportion of representatives of the genera Streptosporangium and Saccharotrix also increased. The treatment of soil samples with 3-ms electric pulses at a field intensity of 16 kV/cm resulted in highly selective isolation of representatives of the genus Nocardioides.
阐述了一种从土壤中选择性分离放线菌的新方法，该方法涉及用电脉冲对土壤样品进行预处理。在12 kV/cm²的场强下用3毫秒电脉冲处理土壤，导致分离出的稀有放线菌代表比例显著提高（平均增加了三倍）。发现这种处理方式对于选择性分离游动放线菌属、小单孢菌属、马杜拉放线菌属和小四孢菌属的代表最为有效。链孢囊菌属和糖丝菌属的代表比例也有所增加。在16 kV/cm的场强下用3毫秒电脉冲处理土壤样品，导致对类诺卡氏菌属的代表进行了高度选择性的分离。
