Wang Fun-In, Kuo Min-Liang, Shun Chia-Tung, Ma Yee-Chung, Wang Jung-Der, Ueng Tzuu-Huei
Department of Veterinary Medicine, College of Agriculture, National Taiwan University, Taipei.
J Toxicol Environ Health A. 2002 Feb;65(3-4):279-91. doi: 10.1080/15287390252800864.
The aim of this study was to determine the chronic toxicity of a mixture of chlorinated alkanes and alkenes (CA) consisting of chloroform, 1,1-dichloroethane, 1,1-dichloroethylene, 1,1,1-trichloroethane, trichloroethylene, and tetrachloroethylene. These chlorinated organic solvents were present in the underground water near an electronic appliances manufactory in Taoyuan, Taiwan. Male and female weanling ICR mice were treated with low-, medium-, and high-dose CA mixtures in drinking water for 16 and 18 mo, respectively. A significant number of male mice treated with the high-dose CA mixture developed tail alopecia and deformation, which was not prominent in CA-treated female mice. Medium- and high-dose CA mixtures induced marginal increases of liver and lung weights, blood urea nitrogen, and serum creatinine levels in male mice. In female mice, the high-dose CA mixture increased liver, kidney, and uterus and ovary total weights, without affecting serum biochemistry parameters. CA mixtures had no effects on the total glutathione content or the level of glutathione S-transferase activity in the livers and kid- neys of male and female mice. Treatments with CA mixtures produced a trend of increasing frequency of hepatocelluar neoplasms in male mice, compared to male and female controls and CA-treated female mice. The high-dose CA mixture induced a significantly higher incidence of mammary adenocarcinoma in female mice. The calculated odds ratios of mammary adenocarcinoma in female mice induced by low-, medium-, and high-dose CA mixtures were 1.14, 1.37, and 3.53 times that of the controls, respectively. The low-dose CA mixture induced a higher incidence of cysts and inflammation in and around the ovaries. This study has demonstrated that the CA mixture is a potential carcinogen to male and female mice. These animal toxicology data may be important in assessing the health effects of individuals exposed to the CA mixture.
本研究的目的是确定由氯仿、1,1 - 二氯乙烷、1,1 - 二氯乙烯、1,1,1 - 三氯乙烷、三氯乙烯和四氯乙烯组成的氯代烷烃和烯烃混合物（CA）的慢性毒性。这些氯代有机溶剂存在于台湾桃园一家电子电器制造厂附近的地下水中。雄性和雌性断乳ICR小鼠分别用低、中、高剂量的CA混合物饮水处理16个月和18个月。用高剂量CA混合物处理的大量雄性小鼠出现尾部脱毛和变形，而在经CA处理的雌性小鼠中不明显。中、高剂量的CA混合物使雄性小鼠的肝脏和肺重量、血尿素氮和血清肌酐水平略有增加。在雌性小鼠中，高剂量CA混合物增加了肝脏、肾脏、子宫和卵巢的总重量，但不影响血清生化参数。CA混合物对雄性和雌性小鼠肝脏和肾脏中的总谷胱甘肽含量或谷胱甘肽S - 转移酶活性水平没有影响。与雄性和雌性对照以及经CA处理的雌性小鼠相比，用CA混合物处理使雄性小鼠肝细胞肿瘤的发生频率呈上升趋势。高剂量CA混合物使雌性小鼠乳腺腺癌的发生率显著升高。低、中、高剂量CA混合物诱导雌性小鼠乳腺腺癌的计算优势比分别是对照组的1.14倍、1.37倍和3.53倍。低剂量CA混合物使卵巢内及周围囊肿和炎症的发生率升高。本研究表明，CA混合物对雄性和雌性小鼠是一种潜在致癌物。这些动物毒理学数据对于评估接触CA混合物个体的健康影响可能很重要。
