Ciarmela Pasquapina, Potenza Lucia, Cucchiarini Luigi, Zeppa Sabrina, Stocchi Vilberto
Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino, PU, Italy.
Microbiol Res. 2002;157(1):69-74. doi: 10.1078/0944-5013-00132.
PCR amplification of the complete intergenic spacer region (IGS) of the Tuber borchii nuclear ribosomal repeat was obtained using universal primers CNL 12 and NS1rev. In order to improve amplification yield a specific primer, T1, was selected from a partial sequence of the IGS product. IGS diversity was characterized both at the intraindividual and intraspecific level. The results obtained at the intraindividual level showed 10% varying repeats on ten screened colonies, while at the intraspecific level the IGS polymorphism was evident as difference in length amplification between mycelial strains and fruit bodies: 3.5 kb and 2 kb respectively.
