博氏块菌核糖体DNA基因间隔区的结构分析

Structural analysis of the rDNA intergenic spacer of Tuber borchii.

作者信息

Ciarmela Pasquapina, Potenza Lucia, Zeppa Sabrina, Cucchiarini Luigi, Stocchi Vilberto

机构信息

Istituto di Chimica Biologica Giorgio Fornaini, Universit degli Studi di Urbino, Via A. Saffi 2, 61029 Urbino (PU), Italy.

出版信息

J Biomol Struct Dyn. 2002 Feb;19(4):701-8. doi: 10.1080/07391102.2002.10506776.

DOI:10.1080/07391102.2002.10506776

PMID:11843631

Abstract

The sequence and characterisation of the entire nuclear rDNA intergenic spacer (IGS) for the genus Tuber are presented. Sequence analyses showed that the organisation of the Tuber borchii rDNA IGS is typical of rDNA spacers, consisting of a central repetitive region and flanking unique sequences on either side. Direct repeats, symmetry elements, tandem repeats and possible areas of recombination were found. The putative ends of the 25S and 17S rDNA were identified. The presence of 5S rDNA in the IGS region was excluded.

摘要

本文展示了块菌属整个核糖体DNA基因间隔区（IGS）的序列及特征。序列分析表明，博氏块菌核糖体DNA基因间隔区的组织形式是核糖体DNA间隔区的典型形式，由一个中央重复区域和两侧的侧翼独特序列组成。发现了直接重复序列、对称元件、串联重复序列以及可能的重组区域。确定了25S和17S核糖体DNA的假定末端。排除了IGS区域中5S核糖体DNA的存在。