Schuch W, Loening U E
Biochem J. 1975 Jul;149(1):17-22. doi: 10.1042/bj1490017.
The 23S rRNA of Agrobacterium tumefaciens contains at least two nicks which result in the formation of RNA components with mol.wts. of 0.52 X 10(6) and 0.48 X 10(6). Thus under the usual conditions of extraction and analysis, no 23S rRNA was recovered from the bacterium. The experiments show that 23S rRNA is synthesized as a continuous chain, in which one or two nicks are formed almost immediately near the ends of the molecule and an additional nick in the middle at a later time.
根癌土壤杆菌的23S rRNA至少含有两个切口,这导致形成分子量分别为0.52×10⁶和0.48×10⁶的RNA组分。因此,在通常的提取和分析条件下,未从该细菌中回收23S rRNA。实验表明,23S rRNA是以连续链的形式合成的,其中在分子末端附近几乎立即形成一个或两个切口,随后在中间形成一个额外的切口。