Rozier C, Rocipon M, Mache R
J Mol Evol. 1979 Nov;13(4):271-9. doi: 10.1007/BF01731367.
The in vivo fragmentation of the plastid rRNA from plants situated at different places in the evolutionary sclae, with the exception of Algae, was analysed by electrophoresis using fully denaturing conditions. This fragmentation corresponds to an in vivo post-maturation. It exists only in some bacteria and is not random. Five main groups of fragments with the following real molecular weights (Mr) are found in 23 S: ca 0.9 x 10(6); 0.7 x 10(6); 0.45 x 10(6); 0.35 x 10(6) and 0.15 x 10(6). The existence of a large fragment (Mr = 0.9 x 10(6)) corresponds to a primitive type of fragmentation found in some archaic plants. Dicotyledons and several other groups have the same pattern of 23 S fragmentation, often comprising all the fragments mentioned above, whilst Graminaceae (Monocotyledons) constitute a special group with a very predominant 0.35 x 10(6) dalton fragment and the absence of the 0.45 x 10(6) dalton fragment. The plastid 16 S rRNA in all plants studied here has a Mr of 0.54 x 10(6) which is smaller than the 16 S of Escherichia coli taken as reference (0.56 x 10(6) dalton).
除藻类外，对处于进化尺度不同位置的植物的质体rRNA进行体内片段化分析，采用完全变性条件下的电泳法。这种片段化对应于体内成熟后过程。它仅存在于某些细菌中，且并非随机发生。在23 S中发现了五组主要片段，其实际分子量（Mr）如下：约0.9×10⁶；0.7×10⁶；0.45×10⁶；0.35×10⁶和0.15×10⁶。存在一个大片段（Mr = 0.9×10⁶）对应于在一些古老植物中发现的原始片段化类型。双子叶植物和其他几个类群具有相同的23 S片段化模式，通常包含上述所有片段，而禾本科（单子叶植物）构成一个特殊类群，其0.35×10⁶道尔顿的片段非常突出，且不存在0.45×10⁶道尔顿的片段。这里研究的所有植物中的质体16 S rRNA的Mr为0.54×10⁶，比作为参考的大肠杆菌的16 S（0.56×10⁶道尔顿）小。
