Hougland A E, Beck J V
Microbios. 1979;24(97-98):151-7.
Cells of Clostridium acidi-urici which were grown in a medium containing uric acid were harvested, disrupted by sonication and centrifuged. After centrifugation the supernatant which served as the cell free extract was used to study the synthesis of serine from 2-14C glycine and formaldehyde. Serine was isolated from the reaction mixture by column chromatography. After identification by paper chromatography, serine was degraded carbon by carbon to locate the position of the labelled carbon. Radioactivity was confined almost exclusively to the alpha carbon of serine which was derived from the alpha carbon of glycine. Formaldehyde, therefore, binds at the alpha carbon of glycine to form serine. Conversion of serine to pyruvate was prevented by adding EDTA to the reaction mixture.
将在含有尿酸的培养基中生长的尿酸梭菌细胞收获，通过超声处理破碎并离心。离心后，用作无细胞提取物的上清液用于研究由2-¹⁴C甘氨酸和甲醛合成丝氨酸的过程。通过柱色谱法从反应混合物中分离出丝氨酸。经纸色谱法鉴定后，将丝氨酸逐碳降解以确定标记碳的位置。放射性几乎完全局限于丝氨酸的α碳，该α碳源自甘氨酸的α碳。因此，甲醛在甘氨酸的α碳处结合形成丝氨酸。通过向反应混合物中加入乙二胺四乙酸（EDTA）来阻止丝氨酸转化为丙酮酸。
