植物组织提取物对甘氨酸和丝氨酸的相互转化作用。
The interconversion of glycine and serine by plant tissue extracts.
作者信息
Cossins E A, Sinha S K
出版信息
Biochem J. 1966 Nov;101(2):542-9. doi: 10.1042/bj1010542.
Abstract
- Extracts prepared from a variety of higher-plant tissues by ammonium sulphate fractionation were shown to catalyse the interconversion of glycine and serine. This interconversion had an absolute requirement for tetrahydrofolate and appeared to favour serine formation. 2. The biosynthesis of serine from glycine was studied in more detail with protein fractionated from 15-day-old wheat leaves. Synthesis of [(14)C]serine from [(14)C]glycine was not accompanied by labelling of glyoxylate, glycollate or formate. 3. The synthesis of serine from glycine was stimulated by additions of formaldehyde, and [(14)C]formaldehyde was readily incorporated into C-3 of serine in the presence of tetrahydrofolate. 4. The results are interpreted as indicating that serine biosynthesis involves a direct cleavage of glycine whereby the alpha-carbon is transferred via N(5)N(10)-methylenetetrahydrofolate to become the beta-carbon of serine.
摘要
- 通过硫酸铵分级分离从多种高等植物组织中制备的提取物显示能催化甘氨酸和丝氨酸的相互转化。这种相互转化绝对需要四氢叶酸，且似乎有利于丝氨酸的形成。2. 用从15日龄小麦叶片中分离的蛋白质对从甘氨酸生物合成丝氨酸进行了更详细的研究。由[¹⁴C]甘氨酸合成[¹⁴C]丝氨酸时，乙醛酸、乙醇酸或甲酸均未被标记。3. 添加甲醛可刺激从甘氨酸合成丝氨酸，并且在四氢叶酸存在下，[¹⁴C]甲醛很容易掺入丝氨酸的C-3位。4. 结果表明，丝氨酸的生物合成涉及甘氨酸的直接裂解，由此α-碳通过N⁵N¹⁰-亚甲基四氢叶酸转移成为丝氨酸的β-碳。
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