Plaza Guillermo, Manzanal Ana I, Fogué Luis, Santón Almudena, Martínez-Montero Juan Carlos, Bellas Carmen
Otolaryngology Department, Ramón y Cajal Hospital, University of Alcalá, Madrid, Spain.
Ann Otol Rhinol Laryngol. 2002 Mar;111(3 Pt 1):210-6. doi: 10.1177/000348940211100304.
We evaluated the association of nasopharyngeal carcinoma (NPC) and Epstein-Barr virus (EBV) in Spanish patients, and studied the expression of EBV products (latent membrane protein-1 [LMP-1] and ZEBRA proteins) by NPC cells and its possible prognostic value. In situ hybridization (ISH) for EBV-encoded nonpolyadenylated RNAs (EBERs) and immunohistochemical expression of LMP-1 and ZEBRA proteins by immunohistochemistry were examined in formalin-fixed, paraffin-embedded NPC specimens from 30 patients, and a survival analysis was done by the Kaplan-Meier method. We detected EBERs by ISH in 96.67% of the NPC cases, and detected expression of LMP-I in 43.33% of the NPC cases and expression of ZEBRA protein in 6.67% of the NPC cases. We conclude that ISH for expression of EBERs is an adequate method for detection of EBV in NPC. LMP-1 is not frequently expressed in NPC cells (43.33%). Most NPC cells carry a latent EBV infection. LMP-1 expression might have worsened the prognosis of NPC in our series.
我们评估了西班牙患者中鼻咽癌（NPC）与爱泼斯坦-巴尔病毒（EBV）的关联，并研究了NPC细胞中EBV产物（潜伏膜蛋白-1 [LMP-1]和ZEBRA蛋白）的表达及其可能的预后价值。在30例患者的福尔马林固定、石蜡包埋的NPC标本中，检测了EBV编码的非多聚腺苷酸化RNA（EBERs）的原位杂交（ISH）以及LMP-1和ZEBRA蛋白的免疫组化表达，并采用Kaplan-Meier法进行了生存分析。我们通过ISH在96.67%的NPC病例中检测到EBERs，在43.33%的NPC病例中检测到LMP-1的表达，在6.67%的NPC病例中检测到ZEBRA蛋白的表达。我们得出结论，检测EBERs表达的ISH是检测NPC中EBV的一种合适方法。LMP-1在NPC细胞中不常表达（43.33%）。大多数NPC细胞携带潜伏性EBV感染。在我们的研究系列中，LMP-1表达可能使NPC的预后恶化。
