Wu J, Khan A A, Shih CY T, Shih D S
Department of Biological Sciences, Louisiana State University and LSU Agricultural Center, Baton Rouge 70803, USA.
DNA Seq. 2001 Dec;12(5-6):447-53. doi: 10.3109/10425170109084473.
Osmotin and osmotin-like proteins (OLPs) are pathogenesis-related (PR) proteins, whose synthesis is normally stimulated upon infection of plants by pathogens. A strawberry genomic clone containing an osmotin-like protein (OLP) gene was isolated and sequenced. This clone contains an open reading frame of 681 nucleotides without any intron. The predicted amino acid sequence of the protein shares high degrees of homology with a number of other OLPs and related proteins, of which several are known to have antifungal activities. Southern hybridization analysis of strawberry genomic DNA suggested that the OLP is coded by a multi-gene family. Results from reverse transcriptase-polymerase chain reaction indicated that this OLP gene is expressed in uninfected strawberry plants.
渗透素和类渗透素蛋白（OLPs）是病程相关（PR）蛋白，其合成通常在植物受到病原体感染时被刺激。分离并测序了一个包含类渗透素蛋白（OLP）基因的草莓基因组克隆。该克隆包含一个681个核苷酸的开放阅读框，没有任何内含子。该蛋白的预测氨基酸序列与许多其他OLPs和相关蛋白具有高度同源性，其中一些已知具有抗真菌活性。草莓基因组DNA的Southern杂交分析表明，OLP由一个多基因家族编码。逆转录聚合酶链反应的结果表明，该OLP基因在未感染的草莓植株中表达。
