Vellicce Gabriel R, Ricci Juan C Díaz, Hernández Lázaro, Castagnaro Atilio P
Departamento de Bioquímica de la Nutrición, Facultad de Bioquímica, Instituto de Química Biológica Dr Bernabé Bloj, Química y Farmacia, Universidad Nacional de Tucumán, Chacabuco 461, CP, 4000, Tucumán, Argentina.
Transgenic Res. 2006 Feb;15(1):57-68. doi: 10.1007/s11248-005-2543-6.
Plants of strawberry (cultivar Pájaro) were transformed with three defense related genes: ch5B, gln2 and ap24 using Agrobacterium tumefaciens. The ch5B gene encodes for a chitinase from Phaseolus vulgaris, while gln2 and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both from Nicotiana tabacum. Sixteen transgenic lines expressing one or a combination of two defense genes were obtained. Phytopathological tests showed that two transgenic lines expressing only the ch5B gene displayed high levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward Colletotrichum acutatum, the etiological agent of the anthracnose disease, was not enhanced in the transgenic plants. These results suggest that the ch5B gene can be used to introduce transgene-mediated resistance to gray mold in strawberry, due to the lack of natural resistance to this disease in the crop.
ch5B、gln2和ap24导入草莓（品种帕亚罗）植株中。ch5B基因编码来自菜豆的一种几丁质酶，而gln2和ap24分别编码来自烟草的一种葡聚糖酶和一种类甜蛋白。获得了16个表达一种或两种防御基因组合的转基因株系。植物病理学测试表明，两个仅表达ch5B基因的转基因株系对灰霉病（灰葡萄孢）表现出高度抗性。这种抗性与外源蛋白CH5B的存在以及叶片中几丁质分解活性的增加相关。然而，转基因植株对炭疽病病原体尖孢炭疽菌的抗性并未增强。这些结果表明，由于草莓作物对这种疾病缺乏天然抗性，ch5B基因可用于在草莓中引入转基因介导的对灰霉病的抗性。
