van Duijvendijk-Matteoli M A, Desmet G M
Biochim Biophys Acta. 1975 Nov 11;408(2):164-9. doi: 10.1016/0005-2728(75)90008-0.
The inhibitory effect of the Cd2+ in the electron transport of the isolated chloroplasts has been observed by measuring the oxygen uptake from the solution and the fluorescence induction. Cd2+ is found to be an inhibitor on the donor side of Photosystem II and its action site, as determined by experiments using hydroxylamine and exogenous Mn, is supposed to be on the water-splitting enzyme itself. Moreover, physicochemical and physiological studies indicate that only the ionic form of Cd is acting at the level of the manganoprotein. It is not possible, from this work, to define precisely in which form Cd is taken up through the thylakoid membranes.
通过测量溶液中的氧气吸收量和荧光诱导，观察到了Cd2+对分离叶绿体电子传递的抑制作用。通过使用羟胺和外源锰的实验确定，Cd2+是光系统II供体侧的抑制剂，其作用位点推测在水裂解酶本身。此外，物理化学和生理学研究表明，只有离子形式的Cd在锰蛋白水平起作用。从这项工作中，无法精确确定Cd是以何种形式通过类囊体膜被吸收的。
