降解植物细胞壁的嗜纤维梭菌纤维小体亚基组成的测定
Determination of subunit composition of Clostridium cellulovorans cellulosomes that degrade plant cell walls.
作者信息
Murashima Koichiro, Kosugi Akihiko, Doi Roy H
机构信息
Section of Molecular, University of California, Davis, California 95616, USA.
出版信息
Appl Environ Microbiol. 2002 Apr;68(4):1610-5. doi: 10.1128/AEM.68.4.1610-1615.2002.
Abstract
Clostridium cellulovorans produces a cellulase enzyme complex (cellulosome). In this study, we isolated two plant cell wall-degrading cellulosomal fractions from culture supernatant of C. cellulovorans and determined their subunit compositions and enzymatic activities. One of the cellulosomal fractions showed fourfold-higher plant cell wall-degrading activity than the other. Both cellulosomal fractions contained the same nine subunits (the scaffolding protein CbpA, endoglucanases EngE and EngK, cellobiohydrolase ExgS, xylanase XynA, mannanase ManA, and three unknown proteins), although the relative amounts of the subunits differed. Since only cellobiose was released from plant cell walls by the cellulosomal fractions, cellobiohydrolases were considered to be key enzymes for plant cell wall degradation.
摘要
食纤维梭菌可产生一种纤维素酶复合物（纤维小体）。在本研究中，我们从食纤维梭菌的培养上清液中分离出两种降解植物细胞壁的纤维小体组分，并测定了它们的亚基组成和酶活性。其中一种纤维小体组分的植物细胞壁降解活性比另一种高四倍。尽管各亚基的相对含量不同，但两种纤维小体组分均含有相同的九个亚基（支架蛋白CbpA、内切葡聚糖酶EngE和EngK、纤维二糖水解酶ExgS、木聚糖酶XynA、甘露聚糖酶ManA以及三种未知蛋白）。由于纤维小体组分仅从植物细胞壁释放出纤维二糖，因此纤维二糖水解酶被认为是植物细胞壁降解的关键酶。
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