Wood Lisa G, Fitzgerald Dominic A, Gibson Peter G, Cooper David M, Garg Manohar L
Discipline of Nutrition and Dietetics, the University of Newcastle, Callaghan, New South Wales, Australia.
Am J Clin Nutr. 2002 Apr;75(4):668-75. doi: 10.1093/ajcn/75.4.668.
Oxidative stress and depleted antioxidant defenses occur in stable cystic fibrosis patients. During acute infection, the balance between oxidants and antioxidants may be further disturbed.
We examined the oxidative stress during acute infection in cystic fibrosis patients by measuring 8-iso-prostaglandin F(2 alpha) (8-iso-PGF(2 alpha)) and antioxidant defenses in relation to dietary intake, fatty acid status, immune function, and clinical status.
Plasma concentrations of total 8-iso-PGF(2 alpha), vitamins E and C, beta-carotene, zinc, selenium, and copper; plasma fatty acid compositions; erythrocyte glutathione concentrations; glutathione peroxidase and superoxide dismutase activity; sputum glutathione and 8-iso-PGF(2 alpha) concentrations; lung function; clinical symptoms; and dietary intake were measured in 15 cystic fibrosis patients before and after 10-14 d of intravenous antibiotic treatment for a pulmonary exacerbation.
After treatment, respiratory status improved (percentage of forced expiratory volume in 1 s: 60 +/- 6% at baseline compared with 74 +/- 7% after treatment, P = 0.01), quality of well-being improved (P = 0.001), and total plasma 8-iso-PGF(2 alpha) concentrations increased from 469 nmol/L at baseline (interquartile range: 373-554 nmol/L) to 565 nmol/L after treatment (interquartile range: 429-689 nmol/L; P = 0.008). Total energy, fat, carbohydrate, and protein intakes per kilogram body weight also increased; however, dietary antioxidant intake was unchanged. Plasma fatty acid concentrations increased after treatment, strongly correlating with plasma 8-iso-PGF(2 alpha) concentrations (r = 0.768, P = 0.001). There were no significant changes in white cell counts or plasma concentrations of vitamins E and C or beta-carotene. Erythrocyte glutathione peroxidase activity was reduced after treatment, whereas there was no significant change in superoxide dismutase activity.
Oxidative stress increased after treatment for pulmonary exacerbations and was strongly linked to increased concentrations of plasma fatty acids. Although intravenous antibiotic therapy and physiotherapy improved lung function within 10-14 d of treatment, the biochemical effects of oxidation continued further. Thus, antioxidant intervention during treatment for and recovery from acute infection in cystic fibrosis should be considered.
氧化应激和抗氧化防御能力下降在稳定期囊性纤维化患者中存在。在急性感染期间，氧化剂和抗氧化剂之间的平衡可能会进一步受到干扰。
我们通过测量8-异前列腺素F（2α）（8-iso-PGF（2α））以及与饮食摄入、脂肪酸状态、免疫功能和临床状态相关的抗氧化防御能力，来研究囊性纤维化患者急性感染期间的氧化应激。
对15名囊性纤维化患者在因肺部病情加重接受10 - 14天静脉抗生素治疗前后，测量血浆中总8-异前列腺素F（2α）、维生素E和C、β-胡萝卜素、锌、硒和铜的浓度；血浆脂肪酸组成；红细胞谷胱甘肽浓度；谷胱甘肽过氧化物酶和超氧化物歧化酶活性；痰液谷胱甘肽和8-异前列腺素F（2α）浓度；肺功能；临床症状；以及饮食摄入情况。
治疗后，呼吸状态改善（一秒用力呼气量百分比：基线时为60±6%，治疗后为74±7%，P = 0.01），健康状况改善（P = 0.001），血浆总8-异前列腺素F（2α）浓度从基线时的469 nmol/L（四分位间距：373 - 554 nmol/L）增加到治疗后的565 nmol/L（四分位间距：429 - 689 nmol/L；P = 0.008）。每千克体重的总能量、脂肪、碳水化合物和蛋白质摄入量也增加；然而，饮食中的抗氧化剂摄入量没有变化。治疗后血浆脂肪酸浓度增加，与血浆8-异前列腺素F（2α）浓度密切相关（r = 0.768，P = 0.001）。白细胞计数、血浆维生素E和C或β-胡萝卜素浓度没有显著变化。治疗后红细胞谷胱甘肽过氧化物酶活性降低，而超氧化物歧化酶活性没有显著变化。
肺部病情加重治疗后氧化应激增加，且与血浆脂肪酸浓度升高密切相关。虽然静脉抗生素治疗和物理治疗在治疗10 - 14天内改善了肺功能，但氧化的生化影响仍持续存在。因此，应考虑在囊性纤维化急性感染治疗及恢复期间进行抗氧化干预。
