Felicetti L, Urbani C
Biochim Biophys Acta. 1975 Dec 4;414(2):146-54. doi: 10.1016/0005-2787(75)90217-8.
A pancreatic deoxyribonuclease preparation, shown to be free of significant ribonuclease activity, inhibits hemoglobin synthesis in a rabbit reticulocytelysate. Preincubation with DNAase (15 min at 25 degrees C) is required to obtain a marked inhibitory effect (nearly 70%). It has been shown that DNAase does not significantly interfere with the elongation or termination steps of translation, but it seems to prevent reinitiation of globin polypeptide chains allowing polysome run-off. In particular, the formation of the 40S/met-tRNAmetf complex is greatly reduced in the DNAase-treated lysate. At the moment the mechanism by which DNAase inhibits initiation is not clear.
一种经检测无显著核糖核酸酶活性的胰腺脱氧核糖核酸酶制剂，可抑制兔网织红细胞裂解液中的血红蛋白合成。需用脱氧核糖核酸酶预孵育（25℃下15分钟）才能获得显著的抑制效果（近70%）。已表明脱氧核糖核酸酶不会显著干扰翻译的延伸或终止步骤，但它似乎会阻止珠蛋白多肽链的重新起始，从而导致多核糖体解聚。特别是，在经脱氧核糖核酸酶处理的裂解液中，40S/甲硫氨酰 - tRNAmetf复合物的形成大大减少。目前，脱氧核糖核酸酶抑制起始的机制尚不清楚。
